| Cytotoxic and cytolytic activity of nonadecafluoro-n-decanoic acid on Acholeplasma laidlawii. | |
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MedLine Citation:
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PMID: 3606106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We studied the interactions between the perfluorinated fatty acid nonadecafluoro-n-decanoic acid (NDFDA) and the cell wall-less procaryote Acholeplasma laidlawii, which were cultured in an identical medium base but with different serum supplements. When grown in mycoplasma media supplemented with PPLO serum fraction (Difco Laboratories, Detroit, Mich.), A. laidlawii was rapidly killed by low concentrations of toxicant (less than 1.0 mM). At higher concentrations (greater than 10 mM), NDFDA treatment appeared to lyse cells. A. laidlawii cells grown in horse serum-supplemented mycoplasma media were both killed and lysed at the same NDFDA concentration (greater than 10 mM). These data suggest that this perfluorinated fatty acid can be cytotoxic and cytolytic to mycoplasmas. Changes in active concentrations occurred in parallel with changes in growth medium serum supplementation, which is known to alter mycoplasma membrane composition. We propose that NDFDA interacts with the membranes of A. laidlawii cells, resulting in cell death or cell lysis or both. |
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Authors:
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A Liss; J C Pfeil; D Levitt |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 53 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 1987 Jun |
Date Detail:
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Created Date: 1987-07-31 Completed Date: 1987-07-31 Revised Date: 2010-09-10 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1236-40 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acholeplasma laidlawii
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analysis,
drug effects*,
growth & development Cell Survival / drug effects Culture Media Decanoic Acids / pharmacology* Densitometry Electrophoresis, Polyacrylamide Gel Fluorocarbons / pharmacology* Peptides / analysis |
| Grant Support | |
ID/Acronym/Agency:
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AM35050/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Decanoic Acids; 0/Fluorocarbons; 0/Peptides; 335-76-2/perfluorodecanoic acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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