| Cytotoxic and apoptotic effects of cobalt and chromium ions on J774 macrophages - Implication of caspase-3 in the apoptotic pathway. | |
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MedLine Citation:
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PMID: 15348346 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to evaluate the cytotoxic and apoptotic effects of cobalt and chromium ions on macrophages in vitro, and analyze the implication of caspase-3 in the apoptotic pathway. J774 mouse macrophages (5 x 10(5) cells/ml) were exposed for up to 24 h to 0-10 ppm Co2+ and 0-500 ppm Cr3+. The cytotoxic effect of ions was measured by Trypan blue exclusion. DNA analysis on agarose gel was used as a specific test for detection of DNA fragmentation into oligonucleosomes that occurs in apoptotic cells. The proteolytic cleavage of poly(ADP-ribose)polymerase (PARP), closely associated with the induction of apoptosis, was also analyzed along with the appearance of the active fragment of caspase-3, implicated in several apoptosis pathways. Results demonstrated that both Co2+ and Cr3+ ions induce macrophage mortality in a dose-dependent manner. However, Co2+ is more toxic inducing a cell mortality up to 28% with only 10 ppm vs. 37% with 500 ppm of Cr3+. DNA analysis demonstrated that both Co2+ and Cr3+ ions induce DNA fragmentation, between 6-10 ppm Co2+ and 250-500 ppm Cr3+ after 24 h incubation. PARP cleavage and the appearance of caspase-3 active fragment were observed after 6 h with both Co+ and Cr3+ ions, with a stronger signal after 24 h and 10 ppm of Co2+ or 500 ppm of Cr3+. In conclusion, this study demonstrates that after 24 h incubation, both Co2+ and Cr3+ ions can induce macrophage mortality, and more specifically apoptosis. The results also suggest that apoptosis occurs via a caspase-3 pathway. However, the relative importance of necrosis and apoptosis and the effects of longer exposure times on the induction of macrophage death by these metal ions remain to be investigated. |
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Authors:
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I Catelas; A Petit; D J Zukor; O L Huk |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of materials science. Materials in medicine Volume: 12 ISSN: 0957-4530 ISO Abbreviation: J Mater Sci Mater Med Publication Date: 2001 Oct-Dec |
Date Detail:
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Created Date: 2004-09-06 Completed Date: 2005-03-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9013087 Medline TA: J Mater Sci Mater Med Country: United States |
Other Details:
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Languages: eng Pagination: 949-53 Citation Subset: - |
Copyright Information:
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Copyright 2001 Kluwer Academic Publishers |
Affiliation:
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Department of Biomedical Engineering, McGill University, Duff Medical Building, 3775 University Street, Montreal, Quebec, Canada, H3A 2B4. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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