Document Detail


Cytotoxic and apoptogenic effect of tea (Camellia sinensis var. assamica) root extract (TRE) and two of its steroidal saponins TS1 and TS2 on human leukemic cell lines K562 and U937 and on cells of CML and ALL patients.
MedLine Citation:
PMID:  16197994     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The anticancer activity of di- and tri-terpenes and other polyphenolic compounds present in tea is already reported. We evaluated the cytotoxic and apoptogenic effect of tea root extract (TRE) and two of its steroidal saponins named as TS1 and TS2, on human cell lines and on cells from leukemic patients. It was found that TRE, TS1 and TS2 significantly decreased cell count and that TRE caused apoptosis, as confirmed morphologically by confocal microscopy and by flow-cytometric analysis using Annexin-V FITC and propidium iodide (PI). Cell count and MTT assay in normal white blood cells (WBC) of healthy volunteers revealed that TRE produced insignificant reduction in cell count and cytotoxicity.
Authors:
Papiya Ghosh; Shila E Besra; Gayatri Tripathi; Smita Mitra; Joseph R Vedasiromoni
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-09-28
Journal Detail:
Title:  Leukemia research     Volume:  30     ISSN:  0145-2126     ISO Abbreviation:  Leuk. Res.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-02-21     Completed Date:  2006-04-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7706787     Medline TA:  Leuk Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  459-68     Citation Subset:  IM    
Affiliation:
Drug Development Division, Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata, 700032 West Bengal, India.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis
DNA Replication / drug effects
Flow Cytometry
Humans
K562 Cells
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology*
Microscopy, Confocal
Plant Extracts / pharmacology*
Plant Roots / chemistry*
Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology*
Saponins / isolation & purification,  pharmacology*
Tea / chemistry*
U937 Cells
Chemical
Reg. No./Substance:
0/Plant Extracts; 0/Saponins; 0/Tea

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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