Document Detail

Cytotoxic and antiproliferative effects of heptaacetyltiliroside on human leukemic cell lines.
MedLine Citation:
PMID:  10576507     Owner:  NLM     Status:  MEDLINE    
The peracetylated derivative of kaempferol-3-O-beta-D-(6''-E-p-coumaroyl) glycopyranoside (tiliroside) (1a) was tested for its cytotoxic and cytostatic activity against several human leukemic cell lines. The significant cytotoxic activity of this derivative, prompted to an additional examination on some of the cell lines used. The effect on the uptake of [3H]thymidine as a marker of DNA synthesis and on the cell proliferation, was investigated as well as the morphology of the cells and the kind of death induced, using the Wright-Giemsa dye and horizontal agarose-gel electrophoresis. Flow cytometric experiments of 1a on some leukemic cell lines was also performed. Compound 1a showed a significant antiproliferative effect as soon as 1 h of continuous incubation at all cell lines tested. Cells were killed, through the process of apoptosis and the appearance of the apoptotic signs was time and dose-dependent, while from the flow cytometric experiments, a synchronisation (through a delay probably in the G(0/1) phase) of the cells seems to take place.
K Dimas; C Demetzos; B Vaos; M Marselos; D Kokkinopoulos
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Leukemia research     Volume:  23     ISSN:  0145-2126     ISO Abbreviation:  Leuk. Res.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-12-08     Completed Date:  1999-12-08     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7706787     Medline TA:  Leuk Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1021-33     Citation Subset:  IM    
Department of Immunology, Hellenic Anticancer Institute, Athens, Greece.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Benzopyrans / pharmacology*
Cell Division / drug effects*
DNA Replication / drug effects
DNA, Neoplasm / drug effects,  metabolism
Electrophoresis, Agar Gel
Flow Cytometry
Leukemia / genetics,  pathology*
Tumor Cells, Cultured
Reg. No./Substance:
0/Antineoplastic Agents; 0/Benzopyrans; 0/DNA, Neoplasm; 0/heptaacetyltiliroside

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