Document Detail

Cytotoxic action of conjugates of alpha-fetoprotein and epidermal growth factor with photoheme, chlorines, and phthalocyanines.
MedLine Citation:
PMID:  10887296     Owner:  NLM     Status:  MEDLINE    
Covalently bound conjugates of alpha-fetoprotein (AFP) and epidermal growth factor (EGF) with photoheme (PH), 3-desvinyl-3-formylchlorine p6 (Chl p6), chlorine e6 (Chl e6), aluminum disulfochloride phthalocyanine (PC(Al)), and cobalt octa-4,5-carboxyphthalocyanine (teraphthal, TP(Co)) were synthesized. Their molar ratios were 1:4 for AFP-cytotoxin conjugates (cf. 1:10 for AFP-TP(Co)) and 1:2 for EGF conjugates (cf. 1:1 for EGF-PC(Al)). Dark toxicity of both protein conjugates with PH, chlorines, and PC(Al) was much lower than their phototoxicity. Studies on phototoxicity demonstrated that PC(Al) conjugates with AFP and EGF and also EGF-Chl p6 were the most effective. The cytotoxic activity (CTA) of AFP-PC(Al) and EGF-Chl p6 was 80% and of EGF-PC(Al) 64% higher than the CTA of the free drugs. Conjugates with TP(Co) were much more toxic on their activation with ascorbic acid (AA): in the presence of AA the CTA of AFP-TP(Co) and of EGF-TP(Co) was 19 and 61.1% higher, respectively, than the CTA of the free TP(Co).
A A Savitsky; N V Gukasova; S G Gumanov; N B Feldman; E A Luk'yanets; A F Mironov; R I Yakubovskaya; S V Lutsenko; S E Severin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry. Biokhimii͡a     Volume:  65     ISSN:  0006-2979     ISO Abbreviation:  Biochemistry Mosc.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-09-19     Completed Date:  2000-09-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376536     Medline TA:  Biochemistry (Mosc)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  732-6     Citation Subset:  IM    
Moscow Research Institute of Medical Ecology, Moscow, 113149, Russia.
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MeSH Terms
Antineoplastic Agents / chemistry,  pharmacology*
Cell Division / drug effects*
Chlorine / chemistry
Epidermal Growth Factor / chemistry,  pharmacology*
Heme / chemistry
Indoles / chemistry
Tumor Cells, Cultured
alpha-Fetoproteins / chemistry,  pharmacology*
Reg. No./Substance:
0/Antineoplastic Agents; 0/Indoles; 0/alpha-Fetoproteins; 14875-96-8/Heme; 574-93-6/phthalocyanine; 62229-50-9/Epidermal Growth Factor; 7782-50-5/Chlorine

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