Document Detail


Cytotoxic T lymphocyte responses to infectious bronchitis virus infection.
MedLine Citation:
PMID:  9782315     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cytotoxic T lymphocyte (CTL) activity to infectious bronchitis virus (IBV) was examined at regular intervals between 3 and 30 days post infection (p.i.). The maximal CTL lysis of target cells infected with IBV with 82% was detected at 10 days p.i. The specific CTL activity began to decrease only after viral loads, which peaked at day 8 p.i. in both kidneys and lungs, started to decline. Therefore, the CTL response correlated with elimination of acute infection. IgM antibody did not appear until day 10 and levels peaked at day 12 p.i. whereas IgG antibody titers were detectable only by day 15 p.i., but continued to increase exponentially until day 30 p.i., the last day examined. IBV specific CTL epitope(s) were mapped within the carboxyl terminal 120 amino acids of nucleocapsid protein. In vivo inoculation of this fragment, as cDNA, induced protection against acute infection. The absence of viral neutralizing epitopes on the nucleocapsid protein would suggest that protection with known CTL eptiope(s) can be induced in the absence of neutralizing antibody.
Authors:
S H Seo; E W Collisson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Advances in experimental medicine and biology     Volume:  440     ISSN:  0065-2598     ISO Abbreviation:  Adv. Exp. Med. Biol.     Publication Date:  1998  
Date Detail:
Created Date:  1999-01-13     Completed Date:  1999-01-13     Revised Date:  2004-08-05    
Medline Journal Info:
Nlm Unique ID:  0121103     Medline TA:  Adv Exp Med Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  455-60     Citation Subset:  IM    
Affiliation:
Department of Veterinary Pathobiology, Texas A & M University, College Station 77843-4467, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Viral / blood
Chick Embryo
Coronavirus Infections / immunology*
Epitope Mapping
Epitopes, T-Lymphocyte / immunology
Immunoglobulin G / blood
Infectious bronchitis virus / genetics,  immunology*
Kidney / pathology,  virology
Lung / pathology,  virology
Nucleocapsid / immunology
Nucleocapsid Proteins*
T-Lymphocytes, Cytotoxic / immunology*
Time Factors
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Epitopes, T-Lymphocyte; 0/Immunoglobulin G; 0/Nucleocapsid Proteins; 0/nucleocapsid protein, Coronavirus

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