Document Detail

Cytotoxic effect of wine polyphenolic extracts and resveratrol against human carcinoma cells and normal peripheral blood mononuclear cells.
MedLine Citation:
PMID:  20482276     Owner:  NLM     Status:  MEDLINE    
Red and white wine polyphenols have been reported to provide substantial health benefits. In this study, the cytotoxic activity of red and white wine polyphenolic extracts and of resveratrol was evaluated against different cancer cell lines--human cervix adenocarcinoma HeLa, human breast adenocarcinoma MDA-MB-361, and human breast carcinoma MDA-MB-453--and normal human peripheral blood mononuclear cells (PBMCs). Qualitative and quantitative compositions of wine polyphenolic extracts obtained by fractional vacuum distillation of corresponding wines were determined using spectrophotometric methods and high-performance liquid chromatography with diode array detection and liquid chromatography with electrospray ionization-time of flight mass spectrometry analysis. It was demonstrated that wine polyphenolic extracts and resveratrol exerted higher cytotoxic activity against HeLa and MDA-MB-453 cells in comparison to MDA-MB-361 cells and unstimulated and stimulated PBMCs. Furthermore, white wine polyphenolic extract exhibited a significantly higher antiproliferative action on cancer cell lines than red wine extract. The presence of condensed or fragmented nuclei in HeLa cells, pretreated with extract of white wine and stained with a mixture of acridine orange and ethidium bromide, pointed to the morphological signs of apoptosis. In addition, HeLa cells in late stages of apoptosis or secondary necrosis were also observed. Results from our study suggest that polyphenolic extracts from red and white wine may have anticarcinogenic potential.
Ivana Matić; Zeljko Zizak; Mladen Simonović; Branislav Simonović; Dejan Godevac; Katarina Savikin; Zorica Juranić
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medicinal food     Volume:  13     ISSN:  1557-7600     ISO Abbreviation:  J Med Food     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-02     Completed Date:  2010-12-06     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  9812512     Medline TA:  J Med Food     Country:  United States    
Other Details:
Languages:  eng     Pagination:  851-62     Citation Subset:  IM    
Institute of Oncology and Radiology of Serbia, Serbia.
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MeSH Terms
Adenocarcinoma / drug therapy*,  immunology,  physiopathology
Cell Line, Tumor
Cell Survival / drug effects
Cells, Cultured
Cytotoxins / analysis,  pharmacology*
Flavonoids / analysis,  pharmacology*
Leukocytes, Mononuclear / drug effects*,  immunology
Phenols / analysis,  pharmacology*
Stilbenes / analysis,  pharmacology*
Uterine Cervical Neoplasms / drug therapy*,  immunology,  physiopathology
Wine / analysis*
Reg. No./Substance:
0/Cytotoxins; 0/Flavonoids; 0/Phenols; 0/Polyphenols; 0/Stilbenes; Q369O8926L/resveratrol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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