Document Detail

Cytotoxic and cell cycle effects induced by two herbal extracts on human cervix carcinoma and human breast cancer cell lines.
MedLine Citation:
PMID:  20170382     Owner:  NLM     Status:  MEDLINE    
In recent times interest has increased in the complementary medicine of cancer patients. Two herbal mixtures were prepared from 17 and 12 plants, respectively. The goal of this study was to examine the in vitro cytotoxic and cell cycle effects of the aqueous-ethanol extracts (Extract 1 and Extract 2) obtained by maceration of the mixtures. The two extracts investigated exhibited significant antiproliferative activity toward two human breast cancer cell lines (MDA-MB-361 and MDA-MB-453) and a human cervix carcinoma cell line (HeLa) with 50% inhibitory concentration (IC(50)) values ranging from 9.92 to 17.38 microL/mL. The extracts did not exert any significant cytotoxicity toward healthy human peripheral blood mononuclear cells. In vitro antitumor activities were accompanied by an important apoptotic fraction of all cell lines after treatment with the extracts. The amount of total phenols was similar in both extracts, whereas the concentration of total tannins was significantly higher in Extract 1. Extract 1 was also found to be a stronger free radical scavenger, with an IC(50) value of 13.4 microg/mL. Both extracts contained rosmarinic acid, while ursolic acid was identified in Extract 2.
Tatjana P Stanojković; Aleksandra Konić-Ristić; Zorica D Juranić; Katarina Savikin; Gordana Zdunić; Nebojsa Menković; Milka Jadranin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medicinal food     Volume:  13     ISSN:  1557-7600     ISO Abbreviation:  J Med Food     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-23     Completed Date:  2010-10-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9812512     Medline TA:  J Med Food     Country:  United States    
Other Details:
Languages:  eng     Pagination:  291-7     Citation Subset:  IM    
Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Belgrade, Serbia.
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MeSH Terms
Adenocarcinoma / drug therapy*
Angiosperms / chemistry
Antineoplastic Agents, Phytogenic / pharmacology,  therapeutic use*
Antioxidants / pharmacology,  therapeutic use
Apoptosis / drug effects
Breast Neoplasms / drug therapy*
Cell Cycle / drug effects*
Cell Line, Tumor
Cell Proliferation / drug effects
Cinnamates / analysis,  pharmacology
Depsides / analysis,  pharmacology
Hela Cells
Inhibitory Concentration 50
Phenols / analysis,  pharmacology
Plant Extracts / chemistry,  pharmacology,  therapeutic use*
Tannins / analysis,  pharmacology
Triterpenes / analysis,  pharmacology
Uterine Cervical Neoplasms / drug therapy*
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Antioxidants; 0/Cinnamates; 0/Depsides; 0/Phenols; 0/Plant Extracts; 0/Tannins; 0/Triterpenes; 537-15-5/rosmarinic acid; 77-52-1/ursolic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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