Document Detail

Cytostatic response of HepG2 to 0.57 MHz electric currents mediated by changes in cell cycle control proteins.
MedLine Citation:
PMID:  21042707     Owner:  NLM     Status:  In-Process    
The capacitive-resistive electric transfer (CRet) therapy is a non-invasive technique that applies electrical currents of 0.4-0.6 MHz to the treatment of musculoskeletal injuries. Although this therapy has proved effective in clinical studies, its interaction mechanisms at the cellular level still are insufficiently investigated. Results from previous studies have shown that the application of CRet currents at subthermal doses causes alterations in cell cycle progression and decreased proliferation in hepatocarcinoma (HepG2) and neuroblastoma (NB69) human cell lines. The aim of the present study was to investigate the antiproliferative response of HepG2 to CRet currents. The results showed that 24-h intermittent treatment with 50 µA/mm(2) current density induced in HepG2 statistically significant changes in expression and activation of cell cycle control proteins p27Kip1 and cyclins D1, A and B1. The chronology of these changes is coherent with that of the alterations reported in the cell cycle of HepG2 when exposed to the same electric treatment. We propose that the antiproliferative effect exerted by the electric stimulus would be primarily mediated by changes in the expression and activation of proteins intervening in cell cycle regulation, which are among the targets of emerging chemical therapies. The capability to arrest the cell cycle through electrically-induced changes in cell cycle control proteins might open new possibilities in the field of oncology.
María Luisa Hernández-Bule; María Antonia Cid; María Angeles Trillo; Jocelyne Leal; Alejandro Ubeda
Related Documents :
21940677 - Pathogenic autoreactive b cells are not negatively selected toward matrix protein colla...
17995027 - Distinguishing cancerous from noncancerous cells through analysis of electrical noise.
6168377 - High gradient magnetic separation of rosette-forming cells.
16106367 - Cellular disorders induced by high magnetic fields.
3148937 - Streptococcus faecalis susceptibility to amiloride depends on medium ph.
15349847 - Hormonal control of somatic cell oocyte interactions during ovarian follicle development.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of oncology     Volume:  37     ISSN:  1791-2423     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1399-405     Citation Subset:  IM    
Servicio Investigación-BEM, IRYCIS, Hospital Ramón y Cajal, 28034 Madrid, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Vascular infarction by subcutaneous application of tissue factor targeted to tumor vessels with NGR-...
Next Document:  Identification and characterization of early growth response 2, a zinc-finger transcription factor, ...