Document Detail

Cytostatic effect of inostamycin, an inhibitor of cytidine 5'-diphosphate 1,2-diacyl-sn-glycerol (CDP-DG): inositol transferase, on oral squamous cell carcinoma cell lines.
MedLine Citation:
PMID:  11448100     Owner:  NLM     Status:  MEDLINE    
Inostamycin, which was recently isolated from Streptomyces sp. MH816-AF15 as an inhibitor of cytidine 5'-diphosphate 1,2-diacyl-sn-glycerol (CDP-DG): inositol transferase, caused a G1-phase accumulation in the cell cycle of small cell lung carcinomas. To investigate whether the cytostatic effect of inostamycin is restricted to lung carcinoma cell lines or applicable to other type of cells, we tested five oral squamous cell carcinoma (SCC) cell lines. Cell growth was suppressed in 62.5--125 ng/ml inostamycin in the culture medium in all oral cancer cell lines tested, with non-viable cells being <1%, indicating inostamycin is cytostatic on SCC cell lines. Decrease in cyclin D1 mRNA and protein expression due to the inostamycin treatment was accompanied by suppression of phosphorylated retinoblastoma susceptibility gene product (pRB-P) levels. Moreover, flow cytometric analysis showed that inostamycin induced an increase in G1/G0 cells (1.2--3.2 fold) over 24 h. These results suggest that inostamycin is a useful agent for tumour dormant cytostatic therapy for oral SCC.
Y Baba; M Tsukuda; I Mochimatsu; S Furukawa; H Kagata; Y Nagashima; S Koshika; M Imoto; Y Kato
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  25     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2001  
Date Detail:
Created Date:  2001-07-12     Completed Date:  2001-10-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  613-20     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Department of Otolaryngology, Yokohama City University School of Medicine, Yokohama 236-0004, Japan.
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MeSH Terms
Antineoplastic Agents / pharmacology*
CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase
Carcinoma, Squamous Cell / drug therapy*,  metabolism,  pathology
Cell Cycle
Cell Division / drug effects
Cyclin D1 / biosynthesis,  genetics
DNA, Neoplasm / analysis
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Flow Cytometry
Furans / pharmacology*
Membrane Proteins
Mouth Neoplasms / drug therapy*,  metabolism,  pathology
Oligonucleotides, Antisense
RNA, Neoplasm / biosynthesis
Retinoblastoma Protein / biosynthesis,  genetics
Transferases (Other Substituted Phosphate Groups) / antagonists & inhibitors*
Tumor Cells, Cultured
Reg. No./Substance:
0/Antineoplastic Agents; 0/DNA, Neoplasm; 0/Furans; 0/Membrane Proteins; 0/Oligonucleotides, Antisense; 0/RNA, Neoplasm; 0/Retinoblastoma Protein; 129905-10-8/inostamycin; 136601-57-5/Cyclin D1; EC 2.7.8.-/Transferases (Other Substituted Phosphate Groups); EC protein, human; EC 3-Phosphatidyltransferase

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