Document Detail


Cytosolic pH Gradients Associated with Tip Growth.
MedLine Citation:
PMID:  17776513     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The presence of a cytosolic pH gradient and its relation to polar tip growth was investigated in rhizoid cells of Pelvetia embryos with the use of pH-sensitive microelectrodes and by ratio imaging. Growing rhizoid cells generated a longitudinal pH gradient in which the apical cytosol was 0.3 to 0.5 units more acidic than the cytosol at the base of the cell. Treatment with a membrane-permeant weak acid, propionic acid, dissipated the cytosolic pH gradient and inhibited growth. The magnitude of the pH gradient correlated well with the rate of tip elongation. The pH gradient spatially superimposed on the cytosolic calcium gradient, and inhibition of calcium fluxes by treatment with lanthanum abolished the pH gradient and inhibited growth.
Authors:
B C Gibbon; D L Kropf
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  263     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  1994 Mar 
Date Detail:
Created Date:  2010-06-08     Completed Date:  2010-07-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1419-21     Citation Subset:  -    
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