Document Detail

Cytosolic Ca2+ homeostasis is a constitutive function of the V-ATPase in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  10991947     Owner:  NLM     Status:  MEDLINE    
The vacuole is the major site of intracellular Ca(2+) storage in yeast and functions to maintain cytosolic Ca(2+) levels within a narrow physiological range via a Ca(2+) pump (Pmc1p) and a H(+)/Ca(2+) antiporter (Vcx1p) driven by the vacuolar H(+)-ATPase (V-ATPase). We examined the function of the V-ATPase in cytosolic Ca(2+) homeostasis by comparing responses to a brief Ca(2+) challenge of a V-ATPase mutant (vma2Delta) and wild-type cells treated with the V-ATPase inhibitor concanamycin A. The kinetics of the Ca(2+) response were determined using transgenic aequorin as an in vivo cytosolic Ca(2+) reporter system. In wild-type cells, the V-ATPase-driven Vcx1p was chiefly responsible for restoring cytosolic Ca(2+) concentrations after a brief pulse. In cells lacking V-ATPase activity, brief exposure to elevated Ca(2+) compromised viability, even when there was little change in the final cytosolic Ca(2+) concentration. vma2Delta cells were more efficient at restoring cytosolic [Ca(2+)] after a pulse than concanamycin-treated wild-type cells, suggesting long term loss of V-ATPase triggers compensatory mechanisms. This compensation was dependent on calcineurin, and was mediated primarily by Pmc1p.
C Forster; P M Kane
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  275     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-01-18     Completed Date:  2001-01-18     Revised Date:  2014-11-13    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  38245-53     Citation Subset:  IM    
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MeSH Terms
Anti-Bacterial Agents / pharmacology
Calcineurin / metabolism
Calcium / metabolism*
Cell Division / drug effects
Cytosol / metabolism
Enzyme Inhibitors / pharmacology
Fungal Proteins / metabolism
Hydrogen-Ion Concentration
Proton-Translocating ATPases / genetics,  metabolism*
Recombinant Proteins / metabolism
Saccharomyces cerevisiae / drug effects,  genetics,  physiology*
Vacuolar Proton-Translocating ATPases*
Grant Support
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Enzyme Inhibitors; 0/Fungal Proteins; 0/Macrolides; 0/Mycotoxins; 0/Recombinant Proteins; 0/Vex1 protein, Pythium vexans; 80890-47-7/concanamycin A; EC; EC 3.6.1.-/Vacuolar Proton-Translocating ATPases; EC ATPases; SY7Q814VUP/Calcium

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