| Cytoskeleton reorganization in influenza hemagglutinin-initiated syncytium formation. | |
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MedLine Citation:
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PMID: 18976631 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Little is known about the mechanisms of cell-cell fusion in development and diseases and, especially, about fusion stages downstream of an opening of nascent fusion pore(s). Earlier works on different cell-cell fusion reactions have indicated that cytoskeleton plays important role in syncytium formation. However, due to complexity of these reactions and multifaceted contributions of cytoskeleton in cell physiology, it has remained unclear whether cytoskeleton directly drives fusion pore expansion or affects preceding fusion stages. Here we explore cellular reorganization associated with fusion pore expansion in syncytium formation using relatively simple experimental system. Fusion between murine embryonic fibroblasts NIH3T3-based cells is initiated on demand by well-characterized fusogen influenza virus hemagglutinin. We uncouple early fusion stages dependent on protein fusogens from subsequent fusion pore expansion stage and establish that the transition from local fusion to syncytium requires metabolic activity of living cells. Effective syncytium formation for cells with disorganized actin and microtubule cytoskeleton argues against hypothesis that cytoskeleton drives fusion expansion. |
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Authors:
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Jean-Philippe Richard; Eugenia Leikina; Leonid V Chernomordik |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Intramural Date: 2008-10-11 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1788 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-02-09 Completed Date: 2009-04-06 Revised Date: 2012-03-07 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 450-7 Citation Subset: IM |
Affiliation:
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Section on Membrane Biology, Laboratory of Cellular and Molecular Biophysics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-1855, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Cytoskeleton / drug effects*, metabolism* Giant Cells / cytology, drug effects*, metabolism* Hemagglutinin Glycoproteins, Influenza Virus / pharmacology* Hydrogen-Ion Concentration Mice NIH 3T3 Cells |
| Grant Support | |
ID/Acronym/Agency:
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Z01 HD001501-16/HD/NICHD NIH HHS; Z99 HD999999/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Hemagglutinin Glycoproteins, Influenza Virus |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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