Document Detail


Cytoskeleton reorganization in influenza hemagglutinin-initiated syncytium formation.
MedLine Citation:
PMID:  18976631     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Little is known about the mechanisms of cell-cell fusion in development and diseases and, especially, about fusion stages downstream of an opening of nascent fusion pore(s). Earlier works on different cell-cell fusion reactions have indicated that cytoskeleton plays important role in syncytium formation. However, due to complexity of these reactions and multifaceted contributions of cytoskeleton in cell physiology, it has remained unclear whether cytoskeleton directly drives fusion pore expansion or affects preceding fusion stages. Here we explore cellular reorganization associated with fusion pore expansion in syncytium formation using relatively simple experimental system. Fusion between murine embryonic fibroblasts NIH3T3-based cells is initiated on demand by well-characterized fusogen influenza virus hemagglutinin. We uncouple early fusion stages dependent on protein fusogens from subsequent fusion pore expansion stage and establish that the transition from local fusion to syncytium requires metabolic activity of living cells. Effective syncytium formation for cells with disorganized actin and microtubule cytoskeleton argues against hypothesis that cytoskeleton drives fusion expansion.
Authors:
Jean-Philippe Richard; Eugenia Leikina; Leonid V Chernomordik
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2008-10-11
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1788     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-09     Completed Date:  2009-04-06     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  450-7     Citation Subset:  IM    
Affiliation:
Section on Membrane Biology, Laboratory of Cellular and Molecular Biophysics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-1855, USA.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Cytoskeleton / drug effects*,  metabolism*
Giant Cells / cytology,  drug effects*,  metabolism*
Hemagglutinin Glycoproteins, Influenza Virus / pharmacology*
Hydrogen-Ion Concentration
Mice
NIH 3T3 Cells
Grant Support
ID/Acronym/Agency:
Z01 HD001501-16/HD/NICHD NIH HHS; Z99 HD999999/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Hemagglutinin Glycoproteins, Influenza Virus
Comments/Corrections

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