Document Detail


Cytoskeletal rearrangements in human red blood cells induced by snake venoms: light microscopy of shapes and NMR studies of membrane function.
MedLine Citation:
PMID:  21933154     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RBCs (red blood cells) circulating through narrow blood capillaries withstand major deformation. The mechanical and chemical stresses commonly exerted on RBCs continue to attract interest for the study of membrane structure and function. Snake venoms are lethal biochemical 'cocktails' that often contain haemotoxins, metalloproteinases, myotoxins, neurotoxins, phosphodiesterases, phospholipases and proteases. We have monitored the effects of 4 snake venoms (Pseudechis guttatus, Oxyuranus scutellatus, Notechis scutatus and Naja kaouthia) on human RBCs using NMR spectroscopy, DIC (differential interference contrast) and confocal light microscopy. RBCs underwent reproducible stomatocytosis, with unusual geographical-like indentations, spherocytosis, followed by rapid lysis. Confocal micrographs using a fluorescent dye linked to phalloidin showed that the change in morphology was associated with the aggregation of actin in the cytoskeleton. (31)P NMR saturation transfer experiments recorded transport of the univalent anion HPA (hypophosphite) on a subsecond time scale, thereby reporting on the function of capnophorin or Band 3 linked to the cytoskeleton; anion-exchange activity was substantially reduced by venom treatment. We propose a molecular-cytological hypothesis for the shape and functional changes that is different from, or supplementary to, the more 'traditional' bilayer-couple hypothesis more often used to account for similar morphological changes invoked by other reagents.
Authors:
Tsz Wai Yau; Rhiannon P Kuchel; Jennifer M S Koh; David Szekely; Peter J Mirtschin; Philip W Kuchel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  36     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-06     Completed Date:  2012-04-06     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  87-97     Citation Subset:  IM    
Copyright Information:
© The Author(s) Journal compilation © 2012 Portland Press Limited
Affiliation:
School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia.
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MeSH Terms
Descriptor/Qualifier:
Actin Cytoskeleton / drug effects
Actins / metabolism
Anion Exchange Protein 1, Erythrocyte / metabolism
Antigens, Human Platelet / metabolism
Cell Membrane / drug effects*,  pathology
Cell Shape
Erythrocytes / cytology,  drug effects*,  pathology
Fluorescent Dyes / chemistry
Hemolysis
Humans
Magnetic Resonance Spectroscopy
Microscopy, Confocal
Phalloidine / chemistry,  pharmacology
Snake Venoms / pharmacology*
Chemical
Reg. No./Substance:
0/Actins; 0/Anion Exchange Protein 1, Erythrocyte; 0/Antigens, Human Platelet; 0/Fluorescent Dyes; 0/Snake Venoms; 0/human platelet antigen 1b; 17466-45-4/Phalloidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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