Document Detail


Cytoskeletal asymmetry in Zea mays subsidiary cell mother cells: a monopolar prophase microtubule half-spindle anchors the nucleus to its polar position.
MedLine Citation:
PMID:  16986138     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Double labeling of microtubules and actin filaments revealed that in prophase subsidiary mother cells of Zea mays a monopolar prophase microtubule "half-spindle" is formed, which lines the nuclear hemisphere distal to the inducing guard mother cell. The nuclear hemisphere proximal to the guard mother cell is lined by an F-actin cap, consisting of a cortical F-actin patch and actin filaments originating from it. The microtubules of the "half-spindle" decline from the nuclear surface and terminate to the preprophase microtubule band. After disintegration of the latter, a bipolar metaphase spindle is organized. The polar F-actin cap persists during mitosis and early cytokinesis, extending to the chromosomes and the subsidiary cell daughter nucleus. In oryzalin treated subsidiary mother cells the prophase nuclei move away from the polar site. Cytochalasin B and latrunculin-B block the polar migration of subsidiary mother cell nuclei, but do not affect those already settled to the polar position. The prophase nuclei of latrunculin-B treated subsidiary mother cells are globally surrounded by microtubules, while the division plane of latrunculin-B treated subsidiary mother cells is misaligned. The prophase nuclei of brick 1 mutant Zea mays subsidiary mother cells without F-actin patch are also globally surrounded by microtubules. The presented data show that the prophase microtubule "half-spindle"-preprophase band complex anchors the subsidiary mother cell nucleus to the polar cell site, while the polar F-actin cap stabilizes the one metaphase spindle pole proximal to the inducing guard mother cell.
Authors:
Emmanuel Panteris; Panagiotis Apostolakos; Basil Galatis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell motility and the cytoskeleton     Volume:  63     ISSN:  0886-1544     ISO Abbreviation:  Cell Motil. Cytoskeleton     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-17     Completed Date:  2006-11-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8605339     Medline TA:  Cell Motil Cytoskeleton     Country:  United States    
Other Details:
Languages:  eng     Pagination:  696-709     Citation Subset:  IM    
Copyright Information:
(c) 2006 Wiley-Liss, Inc.
Affiliation:
Department of Botany, Faculty of Biology, University of Athens, Athens, Greece.
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MeSH Terms
Descriptor/Qualifier:
Bicyclo Compounds, Heterocyclic / pharmacology
Cell Movement / drug effects
Cell Nucleus / drug effects,  physiology*
Cytochalasin B / pharmacology
Cytoskeleton / drug effects,  physiology*
Dinitrobenzenes / pharmacology
Microtubule-Associated Proteins / physiology*
Microtubules / physiology*
Plant Leaves / cytology,  drug effects
Plant Proteins / genetics,  physiology
Prophase / physiology*
Sulfanilamides / pharmacology
Thiazolidines / pharmacology
Triticum / cytology,  physiology
Zea mays / cytology*,  drug effects,  physiology
Chemical
Reg. No./Substance:
0/Bicyclo Compounds, Heterocyclic; 0/Dinitrobenzenes; 0/Microtubule-Associated Proteins; 0/Plant Proteins; 0/Sulfanilamides; 0/Thiazolidines; 14930-96-2/Cytochalasin B; 19044-88-3/oryzalin; 76343-94-7/latrunculin B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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