| Cytosine arabinoside potentiates the apoptotic effect of bendamustine on several B- and T-cell leukemia/lymphoma cells and cell lines. | |
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MedLine Citation:
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PMID: 22530665 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Abstract Bendamustine and cytosine arabinoside (ara-c) are commonly used cytotoxic agents with unique mechanisms of action. We have previously reported a striking additive cytotoxic effect of the consecutive combination of bendamustine and ara-c in mantle-cell lymphoma (MCL) cell lines. In the present study, cell lines of follicular lymphoma (DOHH-2), chronic lymphocytic leukemia/lymphoma (EHEB), diffuse large B-cell lymphoma (SU-DHL-4), T-cell leukemia/lymphoma (JURKAT and KARPAS-299), and MCL (JEKO-1 and GRANTA-519) were exposed to the two single drugs or combined with them simultaneously and consecutively. Peripheral blood chronic lymphocytic leukemia (CLL) B-cells from five patients were also analyzed. Apoptosis, cell proliferation/metabolic activity and mitochondrial damage were evaluated. The combination index (CI) was used to assess the synergy between the drugs. Bendamustine exhibited a relevant cytotoxic effect that was dose- and time-dependent, except for SU-DHL-4 and T-cell lymphoma cells. The addition of ara-c after bendamustine significantly potentiated the single drugs cytotoxic effect of bendamustine on all cell lines, including 17p- CLL B-cells, JURKAT and SU-DHL-4, the latter presenting the higher synergism (CI<0.01). Bendamustine and ara-c are highly synergistic on T- and B-cell lymphoma cell and cell lines, similarly to MCL, overcoming the resistance to the single agents. |
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Authors:
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Silvia Castegnaro; Carlo Visco; Katia Chieregato; Martina Bernardi; Elena Albiero; Cristina Zanon; Domenico Madeo; Francesco Rodeghiero |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-25 |
Journal Detail:
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Title: Leukemia & lymphoma Volume: - ISSN: 1029-2403 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9007422 Medline TA: Leuk Lymphoma Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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