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Cytosine arabinoside potentiates the apoptotic effect of bendamustine on several B- and T-cell leukemia/lymphoma cells and cell lines.
MedLine Citation:
PMID:  22530665     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Bendamustine and cytosine arabinoside (ara-c) are commonly used cytotoxic agents with unique mechanisms of action. We have previously reported a striking additive cytotoxic effect of the consecutive combination of bendamustine and ara-c in mantle-cell lymphoma (MCL) cell lines. In the present study, cell lines of follicular lymphoma (DOHH-2), chronic lymphocytic leukemia/lymphoma (EHEB), diffuse large B-cell lymphoma (SU-DHL-4), T-cell leukemia/lymphoma (JURKAT and KARPAS-299), and MCL (JEKO-1 and GRANTA-519) were exposed to the two single drugs or combined with them simultaneously and consecutively. Peripheral blood chronic lymphocytic leukemia (CLL) B-cells from five patients were also analyzed. Apoptosis, cell proliferation/metabolic activity and mitochondrial damage were evaluated. The combination index (CI) was used to assess the synergy between the drugs. Bendamustine exhibited a relevant cytotoxic effect that was dose- and time-dependent, except for SU-DHL-4 and T-cell lymphoma cells. The addition of ara-c after bendamustine significantly potentiated the single drugs cytotoxic effect of bendamustine on all cell lines, including 17p- CLL B-cells, JURKAT and SU-DHL-4, the latter presenting the higher synergism (CI<0.01). Bendamustine and ara-c are highly synergistic on T- and B-cell lymphoma cell and cell lines, similarly to MCL, overcoming the resistance to the single agents.
Authors:
Silvia Castegnaro; Carlo Visco; Katia Chieregato; Martina Bernardi; Elena Albiero; Cristina Zanon; Domenico Madeo; Francesco Rodeghiero
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-25
Journal Detail:
Title:  Leukemia & lymphoma     Volume:  -     ISSN:  1029-2403     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9007422     Medline TA:  Leuk Lymphoma     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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