Document Detail

Cytoprotective effects of a blue light-filtering intraocular lens on human retinal pigment epithelium by reducing phototoxic effects on vascular endothelial growth factor-alpha, Bax, and Bcl-2 expression.
MedLine Citation:
PMID:  19185255     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To compare the possible protective effects of the ultraviolet (UV)-filtering and blue light-filtering SN60AT intraocular lens (IOL) and the untinted UV-filtering SA60AT IOL with regard to light-induced stress on human retinal pigment epithelium (RPE). SETTING: Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany. METHODS: Primary human RPE cells were exposed to white light, and a tinted or untinted IOL was placed in the light beam. After 15 to 60 minutes of irradiation, cell viability was determined by a colorimetric test (tetrazolium dye-reduction assay) and a microscopic live/dead assay. The expression of vascular endothelial growth factor-alpha (VEGF-alpha), Bax, and Bcl-2 and their mRNA was determined by reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting. RESULTS: Without an IOL, white-light exposure decreased cell viability compared with the decrease with the nonirradiated control in a time-dependent manner. Light-induced cell death was significantly reduced by both the tinted IOL and untinted IOL. The combined UV and blue-light filtering attenuated light-induced cell damage significantly more than UV filtering alone. Results of RT-PCR and Western blotting showed a significant time-dependent decrease in Bcl-2 and increase in Bax and VEGF-alpha that were significantly less with the tinted IOL than with the untinted IOL. CONCLUSIONS: Both IOLs reduced light-induced RPE damage. The UV- and blue light-filtering IOL reduced damage more than the conventional IOL. This supports the hypothesis that blue light-filtering IOLs may prevent retinal damage in clinical use.
Marcus Kernt; Aljoscha S Neubauer; Raffael Liegl; Kirsten H Eibl; Claudia S Alge; Carlo A Lackerbauer; Michael W Ulbig; Anselm Kampik
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cataract and refractive surgery     Volume:  35     ISSN:  1873-4502     ISO Abbreviation:  J Cataract Refract Surg     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-05-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8604171     Medline TA:  J Cataract Refract Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  354-62     Citation Subset:  IM    
Department of Ophthalmology, Ludwig Maximilian University, Munich, Germany.
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MeSH Terms
Blotting, Western
Cell Survival
Cells, Cultured
Gene Expression
Lenses, Intraocular*
Microscopy, Phase-Contrast
Middle Aged
Proto-Oncogene Proteins c-bcl-2 / genetics*,  metabolism
RNA, Messenger / metabolism
Radiation Protection / instrumentation*
Retinal Pigment Epithelium / metabolism,  radiation effects*
Reverse Transcriptase Polymerase Chain Reaction
Ultraviolet Rays
Vascular Endothelial Growth Factor A / genetics*,  metabolism
bcl-2-Associated X Protein / genetics*,  metabolism
Reg. No./Substance:
0/BAX protein, human; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/VEGFA protein, human; 0/Vascular Endothelial Growth Factor A; 0/bcl-2-Associated X Protein

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