Document Detail

Cytoprotective effect of reduced glutathione in hydrogen peroxide-induced endothelial cell injury.
MedLine Citation:
PMID:  1351288     Owner:  NLM     Status:  MEDLINE    
The kinetic effects of hydrogen peroxide (H2O2) on cultured endothelial cells isolated from bovine carotid artery were studied. The cytoprotective effects of glutathione (GSH) on H2O2-induced cell injury were also investigated. H2O2-induced a dose- and time-dependent cell injury in cultured endothelial cells. H2O2-induced cell injury was blocked by simultaneous treatment by catalase, but not by superoxide dismutase. H2O2 also induced endogenous PGI2 biosynthesis, and the maximum PGI2 production was reached after 1 h treatment. Stimulation of PGI2 production was parallel with arachidonate release from H2O2-treated cells. However the prostaglandin biosynthesis enzyme activity in cells was inhibited by H2O2 treatment. When the cells were treated with GSH, the intracellular GSH reached a plateau after 3 h treatment. Both H2O2-induced cell injury and PGI2 production were significantly inhibited by the 3 h pretreatment with GSH. The cytoprotective effect of GSH was completely inhibited by buthionine sulfoximine which is a specific inhibitor of gamma-glutamylcysteine synthetase. The results indicate that the cytoprotective effect of GSH on H2O2-induced cell injury in cultured bovine carotid artery endothelial cells depends on the increase in intracellular GSH content.
S H Chen; H L Wu; M T Lin; C J Jen; L Y Wing; H Y Lei; C J Tsao; W C Chang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Prostaglandins, leukotrienes, and essential fatty acids     Volume:  45     ISSN:  0952-3278     ISO Abbreviation:  Prostaglandins Leukot. Essent. Fatty Acids     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-07-13     Completed Date:  1992-07-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802730     Medline TA:  Prostaglandins Leukot Essent Fatty Acids     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  299-305     Citation Subset:  IM    
Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China.
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MeSH Terms
Buthionine Sulfoximine
Catalase / pharmacology
Cells, Cultured
Endothelium, Vascular / drug effects*,  injuries,  metabolism
Epoprostenol / biosynthesis
Glutamate-Cysteine Ligase / antagonists & inhibitors
Glutathione / biosynthesis,  pharmacology*
Hydrogen Peroxide / toxicity*
Methionine Sulfoximine / analogs & derivatives,  pharmacology
Reg. No./Substance:
1982-67-8/Methionine Sulfoximine; 35121-78-9/Epoprostenol; 5072-26-4/Buthionine Sulfoximine; 70-18-8/Glutathione; 7722-84-1/Hydrogen Peroxide; EC; EC Ligase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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