Document Detail


Cytoprotective effect of reduced glutathione in hydrogen peroxide-induced endothelial cell injury.
MedLine Citation:
PMID:  1351288     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The kinetic effects of hydrogen peroxide (H2O2) on cultured endothelial cells isolated from bovine carotid artery were studied. The cytoprotective effects of glutathione (GSH) on H2O2-induced cell injury were also investigated. H2O2-induced a dose- and time-dependent cell injury in cultured endothelial cells. H2O2-induced cell injury was blocked by simultaneous treatment by catalase, but not by superoxide dismutase. H2O2 also induced endogenous PGI2 biosynthesis, and the maximum PGI2 production was reached after 1 h treatment. Stimulation of PGI2 production was parallel with arachidonate release from H2O2-treated cells. However the prostaglandin biosynthesis enzyme activity in cells was inhibited by H2O2 treatment. When the cells were treated with GSH, the intracellular GSH reached a plateau after 3 h treatment. Both H2O2-induced cell injury and PGI2 production were significantly inhibited by the 3 h pretreatment with GSH. The cytoprotective effect of GSH was completely inhibited by buthionine sulfoximine which is a specific inhibitor of gamma-glutamylcysteine synthetase. The results indicate that the cytoprotective effect of GSH on H2O2-induced cell injury in cultured bovine carotid artery endothelial cells depends on the increase in intracellular GSH content.
Authors:
S H Chen; H L Wu; M T Lin; C J Jen; L Y Wing; H Y Lei; C J Tsao; W C Chang
Related Documents :
1351288 - Cytoprotective effect of reduced glutathione in hydrogen peroxide-induced endothelial c...
20149858 - Mg132, a proteasome inhibitor decreased the growth of calu-6 lung cancer cells via apop...
20716928 - Transient glutathione depletion determines terminal differentiation in hl-60 cells.
8900398 - Direct reaction of h2o2 with sulfhydryl groups in hl-60 cells: zinc-metallothionein and...
7691798 - Stringency and relaxation among the halobacteria.
21402478 - Synthesis of a new 4-aza-2,3-didehydropodophyllotoxin analogues as potent cytotoxic and...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Prostaglandins, leukotrienes, and essential fatty acids     Volume:  45     ISSN:  0952-3278     ISO Abbreviation:  Prostaglandins Leukot. Essent. Fatty Acids     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-07-13     Completed Date:  1992-07-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802730     Medline TA:  Prostaglandins Leukot Essent Fatty Acids     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  299-305     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Buthionine Sulfoximine
Catalase / pharmacology
Cells, Cultured
Endothelium, Vascular / drug effects*,  injuries,  metabolism
Epoprostenol / biosynthesis
Glutamate-Cysteine Ligase / antagonists & inhibitors
Glutathione / biosynthesis,  pharmacology*
Hydrogen Peroxide / toxicity*
Methionine Sulfoximine / analogs & derivatives,  pharmacology
Chemical
Reg. No./Substance:
1982-67-8/Methionine Sulfoximine; 35121-78-9/Epoprostenol; 5072-26-4/Buthionine Sulfoximine; 70-18-8/Glutathione; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.6/Catalase; EC 6.3.2.2/Glutamate-Cysteine Ligase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Congestive heart failure with normal ejection fraction. A different mechanism requiring different th...
Next Document:  Conformational coupling in DNA polymerase information transfer.