| Cytoprotective effect of chlorogenic acid against α-synuclein-related toxicity in catecholaminergic PC12 cells. | |
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MedLine Citation:
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PMID: 22962530 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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Parkinson's disease is a major neurodegenerative disease involving the selective degeneration of dopaminergic neurons and α-synuclein containing Lewy bodies formation in the substantia nigra. Although α-synuclein is a key molecule for both dopaminergic neuron death and the formation of inclusion bodies, the mechanism of α-synuclein induction of Parkinson's disease-related pathogenesis is not understood. In the present study, we found that the interaction between dopamine and α-synuclein requires the oxidation of dopamine. Furthermore, we examined the protective effect of chlorogenic acid, a major polyphenol contained in coffee, against α-syn and dopamine-related toxicity. Chlorogenic acid inhibits several DA/α-synuclein-related phenomenon, including the oxidation of dopamine, the interaction of oxidized dopamine with α-synuclein, and the oligomerization of α-synuclein under dopamine existing conditions in vitro. Finally, we showed that the cytoprotective effect against α-synuclein-related toxicity in PC12 cells that can be controlled by the Tet-Off system. Although the induction of α-synuclein in catecholaminergic PC12 cells causes a decrease in cell viability, chlorogenic acid rescued this cytotoxicity significantly in a dose dependent manner. These results suggest that the interaction of oxidized DA with α-synuclein may be a novel therapeutic target for Parkinson's disease, and polyphenols, including chlorogenic acid, are candidates as protective and preventive agents for Parkinson's disease onset. |
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Authors:
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Mari Teraoka; Kazuhiro Nakaso; Chiaki Kusumoto; Satoshi Katano; Naoko Tajima; Atsushi Yamashita; Teppei Zushi; Satoru Ito; Tatsuya Matsura |
Publication Detail:
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Type: Journal Article Date: 2012-04-06 |
Journal Detail:
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Title: Journal of clinical biochemistry and nutrition Volume: 51 ISSN: 1880-5086 ISO Abbreviation: J Clin Biochem Nutr Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-09-10 Completed Date: 2012-09-11 Revised Date: 2013-03-08 |
Medline Journal Info:
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Nlm Unique ID: 8700907 Medline TA: J Clin Biochem Nutr Country: Japan |
Other Details:
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Languages: eng Pagination: 122-7 Citation Subset: - |
Affiliation:
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Division of Medical Biochemistry, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine, Tottori University, 86, Nishi-cho, Yonago 683-8503, Japan. |
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