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Cytoprotective effect of chlorogenic acid against α-synuclein-related toxicity in catecholaminergic PC12 cells.
MedLine Citation:
PMID:  22962530     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Parkinson's disease is a major neurodegenerative disease involving the selective degeneration of dopaminergic neurons and α-synuclein containing Lewy bodies formation in the substantia nigra. Although α-synuclein is a key molecule for both dopaminergic neuron death and the formation of inclusion bodies, the mechanism of α-synuclein induction of Parkinson's disease-related pathogenesis is not understood. In the present study, we found that the interaction between dopamine and α-synuclein requires the oxidation of dopamine. Furthermore, we examined the protective effect of chlorogenic acid, a major polyphenol contained in coffee, against α-syn and dopamine-related toxicity. Chlorogenic acid inhibits several DA/α-synuclein-related phenomenon, including the oxidation of dopamine, the interaction of oxidized dopamine with α-synuclein, and the oligomerization of α-synuclein under dopamine existing conditions in vitro. Finally, we showed that the cytoprotective effect against α-synuclein-related toxicity in PC12 cells that can be controlled by the Tet-Off system. Although the induction of α-synuclein in catecholaminergic PC12 cells causes a decrease in cell viability, chlorogenic acid rescued this cytotoxicity significantly in a dose dependent manner. These results suggest that the interaction of oxidized DA with α-synuclein may be a novel therapeutic target for Parkinson's disease, and polyphenols, including chlorogenic acid, are candidates as protective and preventive agents for Parkinson's disease onset.
Authors:
Mari Teraoka; Kazuhiro Nakaso; Chiaki Kusumoto; Satoshi Katano; Naoko Tajima; Atsushi Yamashita; Teppei Zushi; Satoru Ito; Tatsuya Matsura
Publication Detail:
Type:  Journal Article     Date:  2012-04-06
Journal Detail:
Title:  Journal of clinical biochemistry and nutrition     Volume:  51     ISSN:  1880-5086     ISO Abbreviation:  J Clin Biochem Nutr     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-10     Completed Date:  2012-09-11     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  8700907     Medline TA:  J Clin Biochem Nutr     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  122-7     Citation Subset:  -    
Affiliation:
Division of Medical Biochemistry, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine, Tottori University, 86, Nishi-cho, Yonago 683-8503, Japan.
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