Document Detail


Cytoplasmic volume condensation is an integral part of mitosis.
MedLine Citation:
PMID:  17581282     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell growth and osmotic volume regulation are undoubtedly linked to the progression of the cell cycle as with each division, a newly generated cell must compensate for loss of half of its volume to its sister cell. The extent to which size influences cell cycle decisions, however, is controversial in mammalian cells. Further, a mechanism by which cells can monitor and therefore regulate their size has not been fully elucidated. Despite an ongoing debate, there have been few studies which directly address the question in single cell real-time experiments. In this study we used fluorescent time-lapse imaging to quantitatively assess volume in individual spontaneously dividing cells throughout the cell cycle. Together with biophysical studies, these establish that the efflux of salt and water brings about a condensation of cytoplasmic volume as glioma cells progress through mitosis. As cells undergo this pre-mitotic condensation (PMC) they approach a preferred cell volume preceding each division. This is functionally linked to chromatin condensation, suggesting that PMC plays an integral role in mitosis.
Authors:
Christa W Habela; Harald Sontheimer
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-04-27
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  6     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-11     Completed Date:  2007-09-04     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1613-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Division
Cell Membrane / physiology
Cell Size*
Computer Simulation
Cytokinesis / physiology*
Cytoplasm / physiology*
Humans
Mitosis / physiology*
Models, Biological
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
P50 CA097247/CA/NCI NIH HHS; P50 CA097247-05/CA/NCI NIH HHS; P50-CA97247/CA/NCI NIH HHS; R01 NS031234/NS/NINDS NIH HHS; R01 NS031234-14A1/NS/NINDS NIH HHS; R01 NS036692/NS/NINDS NIH HHS; R01 NS036692-08/NS/NINDS NIH HHS; R01-NS313234/NS/NINDS NIH HHS; R01-NS36692/NS/NINDS NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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