Document Detail


Cytoplasmic requirement for peroxisome biogenesis in Chinese hamster ovary cells.
MedLine Citation:
PMID:  2780559     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hybrids constructed by fusion of wild-type Chinese hamster ovary cells (CHO-K1) to peroxisome-deficient CHO mutants (ZR-78.1) contain normal peroxisomes, demonstrating that the mutation(s) are recessive. "Nuclear hybrids" prepared by fusion of CHO-K1 karyoplasts to mutant ZR-78.1 occasionally fail to regain intact peroxisomes (approximately 1/300 cells). These peroxisome-deficient nuclear hybrids closely resemble the original mutant cells by biochemical criteria, but their modal chromosome number is 36-38, the same as that of CHO hybrids generated from intact cells. When the peroxisome-deficient nuclear hybrids are fused to wild-type cytoplasts, a fraction of the fusion products (at least 70%) continue to propagate normal peroxisomes indefinitely. Peroxisome biogenesis cannot be reinitiated in cells of mutant ZR-78.1 by fusion to wild-type cytoplasts. Our results suggest that a wild-type nucleus by itself is necessary but not sufficient for restoration of normal peroxisome biogenesis and that a cytoplasmic component of wild-type cells, possibly a normal peroxisome, is also required.
Authors:
L A Allen; O H Morand; C R Raetz
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  86     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1989 Sep 
Date Detail:
Created Date:  1989-10-24     Completed Date:  1989-10-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7012-6     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fusion
Cell Line
Cricetinae
Cricetulus
Cytoplasm / physiology
Female
Hybrid Cells / cytology,  physiology
Microbodies / physiology*,  ultrastructure
Mutation
Ovary
Plasmalogens / analysis
Grant Support
ID/Acronym/Agency:
DK 21722/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Plasmalogens
Comments/Corrections

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