Document Detail


Cytoplasmic membrane fluidity measurements on intact living cells of Bacillus subtilis by fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene.
MedLine Citation:
PMID:  3129345     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for determination of membrane fluidity (microviscosity) in Bacillus subtilis cytoplasmic membrane under in vivo conditions is described. The membranes were labelled with the hydrophobic fluorescent probe 1,6-diphenyl-1,3,5-hexatriene during the exponential phase of growth. Fluorescence anisotropy measurements were carried out in an intact cell suspension having absorbance A as high as 0.2-0.3 (corresponding to a cell concentration of 100-300/nL).
Authors:
J Svobodová; P Svoboda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Folia microbiologica     Volume:  33     ISSN:  0015-5632     ISO Abbreviation:  Folia Microbiol. (Praha)     Publication Date:  1988  
Date Detail:
Created Date:  1988-05-31     Completed Date:  1988-05-31     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0376757     Medline TA:  Folia Microbiol (Praha)     Country:  CZECHOSLOVAKIA    
Other Details:
Languages:  eng     Pagination:  1-9     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Faculty of Science, Charles University, Prague.
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MeSH Terms
Descriptor/Qualifier:
Bacillus subtilis / metabolism*
Cell Membrane / metabolism
Diphenylhexatriene
Fluorescence Polarization
Kinetics
Membrane Fluidity*
Protoplasts / metabolism
Thermodynamics
Chemical
Reg. No./Substance:
1720-32-7/Diphenylhexatriene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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