| Cytoplasmic localization and ubiquitination of p21(Cip1) by reactive oxygen species. | |
| | |
MedLine Citation:
|
PMID: 17477906 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Reactive oxygen species were previously shown to trigger p21(Cip1) protein degradation through a proteasome-dependent pathway, however the detailed mechanism of degradation remains to be elucidated. In this report, we showed that p21(Cip1) was degraded at an early phase after low dose H(2)O(2) treatment of a variety of cell types and that preincubation of cells with the antioxidant, N-acetylcysteine, prolonged p21(Cip1) half-life. A mutant p21(Cip1) in which all six lysines were changed to arginines was protected against H(2)O(2) treatment. Direct interaction between p21(Cip1) and Skp2 was elevated in the H(2)O(2)-treated cells. Disruption of the two nuclear export signal (NES) sequences in p21(Cip1), or treatment with leptomycin B blocked H(2)O(2)-induced p21(Cip1) degradation. Altogether, these results demonstrate that reactive oxygen species induce p21(Cip1) degradation through an NES-, Skp2-, and ubiquitin-dependent pathway. |
| | |
Authors:
|
Chae Young Hwang; Ick Young Kim; Ki-Sun Kwon |
Related Documents
:
|
20204766 - Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. 2163466 - Production of reactive oxygen species by tubular epithelial cells in culture. 20809936 - Mast cells and hypoxia drive tissue metaplasia and heterotopic ossification in idiopath... 8454226 - Oxygen radicals and the control of ovarian corpus luteum function. 7849186 - Cyclic and postnatal developmental changes of testin in the rat seminiferous epithelium... 7858146 - Preferred negative geotactic orientation in mobile cells: tetrahymena results. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-04-25 |
Journal Detail:
|
Title: Biochemical and biophysical research communications Volume: 358 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2007 Jun |
Date Detail:
|
Created Date: 2007-05-15 Completed Date: 2007-07-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
|
Languages: eng Pagination: 219-25 Citation Subset: IM |
Affiliation:
|
Laboratory of Cell Signaling, Proteome Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetylcysteine
/
pharmacology Amino Acid Sequence Amino Acid Substitution Animals Antioxidants / pharmacology Cell Line Cyclin-Dependent Kinase Inhibitor p21 / genetics, metabolism* Cytoplasm / metabolism* Fatty Acids, Unsaturated / pharmacology Humans Hydrogen Peroxide / pharmacology Molecular Sequence Data Nuclear Export Signals Reactive Oxygen Species / metabolism* S-Phase Kinase-Associated Proteins / metabolism Ubiquitin / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Fatty Acids, Unsaturated; 0/Nuclear Export Signals; 0/Reactive Oxygen Species; 0/S-Phase Kinase-Associated Proteins; 0/Ubiquitin; 616-91-1/Acetylcysteine; 7722-84-1/Hydrogen Peroxide; 87081-35-4/leptomycin B |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The heterogeneity of epithelial ovarian cancers: reconciling old and new paradigms.
Next Document: Induction of autoantibodies to murine P-glycoprotein: consequences on drug sensitivity in MDR cancer...