Document Detail


Cytoplasmic alpha-fetoprotein functions as a co-repressor in RA-RAR signaling to promote the growth of human hepatoma Bel 7402 cells.
MedLine Citation:
PMID:  19501957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of AFP in the retinoic acid-RAR signaling pathway was investigated in human hepatoma Bel 7402 cells. The results showed that AFP and RAR-beta were co-localized and interacted in cytoplasm. AFP may inhibit translocation of RAR-beta into the nucleus via competitive binding to RAR-beta with ATRA, which was reversed by AFP-siRNA transfection. Our data suggest that the ATRA resistance of Bel 7402 cells is at least in part attributable to their high level of cytoplasmic AFP. Thus, by counteracting the effect of AFP, it may be possible to increase the sensitivity of tumor cells to ATRA.
Authors:
Mengsen Li; Hui Li; Chaoying Li; Liyuan Guo; Han Liu; Sheng Zhou; Xinhua Liu; Zhuo Chen; Shuanglin Shi; Jiang Wei; Michael A McNutt; Gang Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-06
Journal Detail:
Title:  Cancer letters     Volume:  285     ISSN:  1872-7980     ISO Abbreviation:  Cancer Lett.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-20     Completed Date:  2009-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  190-9     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100083, China.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Carcinoma, Hepatocellular / metabolism*
Cell Line, Tumor
Cytoplasm / chemistry,  metabolism
Drug Resistance, Neoplasm / physiology*
Flow Cytometry
Humans
Immunoprecipitation
Liver Neoplasms / metabolism*
Microtubule-Associated Proteins / biosynthesis
Protein Transport / drug effects,  physiology
Receptors, Retinoic Acid / metabolism*
Signal Transduction / drug effects,  physiology
Transfection
Tretinoin / metabolism*,  pharmacology
alpha-Fetoproteins / metabolism*
Chemical
Reg. No./Substance:
0/BIRC5 protein, human; 0/Microtubule-Associated Proteins; 0/Receptors, Retinoic Acid; 0/alpha-Fetoproteins; 302-79-4/Tretinoin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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