Document Detail


Cytopathology by optical methods: spectral cytopathology of the oral mucosa.
MedLine Citation:
PMID:  20142808     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Spectral cytopathology (SCP) is a novel approach for diagnostic differentiation of disease in individual exfoliated cells. SCP is carried out by collecting information on each cell's biochemical composition through an infrared micro-spectral measurement, followed by multivariate data analysis. Deviations from a cell's natural composition produce specific spectral patterns that are exclusive to the cause of the deviation or disease. These unique spectral patterns are reproducible and can be identified and used through multivariate statistical methods to detect cells compromised at the molecular level by dysplasia, neoplasia, or viral infection. In this proof of concept study, a benchmark for the sensitivity of SCP is established by classifying healthy oral squamous cells according to their anatomical origin in the oral cavity. Classification is achieved by spectrally detecting cells with unique protein expressions: for example, the squamous cells of the tongue are the only cell type in the oral cavity that have significant amounts of intracytoplasmic keratin, which allows them to be spectrally differentiated from other oral mucosa cells. Furthermore, thousands of cells from a number of clinical specimens were examined, among them were squamous cell carcinoma, malignancy-associated changes including reactive atypia, and infection by the herpes simplex virus. Owing to its sensitivity to molecular changes, SCP often can detect the onset of disease earlier than is currently possible by cytopathology visualization. As SCP is based on automated instrumentation and unsupervised software, it constitutes a diagnostic workup of medical samples devoid of bias and inconsistency. Therefore, SCP shows potential as a complementary tool in medical cytopathology.
Authors:
Kostas Papamarkakis; Benjamin Bird; Jennifer M Schubert; Milos Miljković; Richard Wein; Kristi Bedrossian; Nora Laver; Max Diem
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-02-08
Journal Detail:
Title:  Laboratory investigation; a journal of technical methods and pathology     Volume:  90     ISSN:  1530-0307     ISO Abbreviation:  Lab. Invest.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-30     Completed Date:  2010-05-17     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  0376617     Medline TA:  Lab Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  589-98     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Chemical Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA. Kpapamar@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Epithelial Cells / pathology
Herpes Labialis / diagnosis,  pathology
Histocytochemistry
Humans
Infrared Rays / diagnostic use
Mouth Mucosa / pathology*
Mouth Neoplasms / diagnosis*,  pathology
Spectrum Analysis / methods*
Grant Support
ID/Acronym/Agency:
CA 090346/CA/NCI NIH HHS; R01 CA090346-06/CA/NCI NIH HHS; R21 CA153148-02/CA/NCI NIH HHS
Comments/Corrections

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