Document Detail

Cytometric analysis of neoplastic transformation of vertebrate cell populations.
MedLine Citation:
PMID:  1268819     Owner:  NLM     Status:  MEDLINE    
Two parameters of neoplastic transformation in spontaneously transforming fibroblasts were examined. One parameter, the predilection for rounding of cells at metaphase, was studied using time-lapse cinephotomicrography and fixed, stained preparations. Ten different cell lines were assayed, including established lines of hamster, rat, and mouse cells, and six rodent cultures of known neoplastic potential as determined by animal injection and tumor production. There was a close correlation between the assumption by cells of a spherical shape at metaphase and their ability to form tumors on injection in syngeneic hosts. The projected area of adherent cells 24 hr after plating over a coverglass was used to assay the transition of these rodent cell populations in culture from nonneoplastic to neoplastic. As the cell population became neoplastic, there was a significant decrease in the mean projected area of the cells. Furthermore, as the cell cultures became capable of producing tumors, the projected area profile of the population shifted proportionally to smaller area classes.
C H Fox; J A Dvorak; K K Sanford
Related Documents :
19596789 - The tumor suppressor functions of p27(kip1) include control of the mesenchymal/amoeboid...
6437669 - Heterogeneity of human colon carcinoma.
4327589 - Transfer of thymidine kinase to thymidine kinaseless l cells by infection with ultravio...
11983939 - Harvesting cells under anchorage-independent cell transformation conditions for biochem...
359129 - Population density as a factor in the evolution of neoplastic cell lines.
6847639 - Differences in polyamine availability and insertion into fibronectins released from nor...
11361019 - Demonstration of the activation of prodrug cb 1954 using human dt-diaphorase mutant q10...
1826959 - Oxygen dependency of tumor cell killing in vitro by light-activated photofrin ii.
6086409 - Molecular studies of the induction of cellular phospholipidosis by cationic amphiphilic...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer research     Volume:  36     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1976 May 
Date Detail:
Created Date:  1976-08-02     Completed Date:  1976-08-02     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1556-61     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Line
Cell Transformation, Neoplastic / pathology*
Cells, Cultured
Clone Cells
Neoplasms, Experimental / etiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Enhancing effect of allopurinol on the induction of bladder cancer in rats by n-[4-(5-nitro-2-furyl)...
Next Document:  Effects of adriamycin on ultrastructure of nucleoli in the heart and liver cells of the rat.