Document Detail

Cytomegalovirus enhance expression of growth factors during the development of chronic allograft nephropathy in rats.
MedLine Citation:
PMID:  15910304     Owner:  NLM     Status:  MEDLINE    
Cytomegalovirus (CMV) accelerates chronic rejection (CRX) in a model of rat kidney allograft. In this model, the expressions of transforming growth factor beta 1 (TGF-beta), platelet-derived growth factor (PDGF)-AA, PDGF-BB and connective tissue growth factor (CTGF) were investigated with and without CMV. Transplantations were performed under immunosuppression. One group of animals was infected with CMV and the other was left uninfected. The grafts were harvested on days 3-60 after transplantation. Growth factor proteins were demonstrated by immunohistochemistry, and mRNAs by in situ hybridization. A significantly more intense and earlier endothelial TGF-beta (2.4 +/- 0.8 vs. 1.0 +/- 0.0; P < 0.05) and PDGF-AA (1.8 +/- 0.4 vs. 1.0 +/- 0.0; P < 0.05) expressions, confirmed by mRNA hybridization, occurred in the CMV group compared with the noninfected group. PDGF-BB appeared in a few inflammatory cells only. In addition CTGF appeared earlier and has more intense in the CMV group (2.5 +/- 0.6 vs. 1.2 +/- 0.5) and the number of CTGF mRNA-positive fibroblasts (57 +/- 9 vs. 3 +/- 4; P < 0.05) was significantly higher. Thus, CMV enhanced expression of TGF-beta1, PDGF-AA and CTGF during the development of CRX.
Kaija Inkinen; Anu Soots; Leena Krogerus; Raisa Loginov; Cathrien Bruggeman; Irmeli Lautenschlager
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transplant international : official journal of the European Society for Organ Transplantation     Volume:  18     ISSN:  0934-0874     ISO Abbreviation:  Transpl. Int.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-05-24     Completed Date:  2005-08-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8908516     Medline TA:  Transpl Int     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  743-9     Citation Subset:  IM    
Department of Virology, Helsinki University Central Hospital and Helsinki University, Helsinki, Finland.
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MeSH Terms
Chronic Disease
Connective Tissue Growth Factor
Cytomegalovirus Infections / metabolism*
Graft Rejection / metabolism*
Immediate-Early Proteins / analysis*,  genetics
Intercellular Signaling Peptides and Proteins / analysis*,  genetics
Kidney / pathology
Kidney Transplantation / adverse effects*
Platelet-Derived Growth Factor / analysis*,  genetics
RNA, Messenger / analysis
Rats, Inbred BN
Transforming Growth Factor beta / analysis*,  genetics
Transplantation, Homologous
Reg. No./Substance:
0/Ctgf protein, rat; 0/Immediate-Early Proteins; 0/Intercellular Signaling Peptides and Proteins; 0/Platelet-Derived Growth Factor; 0/RNA, Messenger; 0/Transforming Growth Factor beta; 139568-91-5/Connective Tissue Growth Factor

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