Document Detail


Cytolytic assessment of hyperacute rejection and production of nuclear transfer embryos using hCD46-transgenic porcine embryonic germ cells.
MedLine Citation:
PMID:  19063773     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human complement regulatory protein hCD46 may reduce the hyperacute rejection (HAR) in pig-to-human xenotransplantation. In this study, an hCD46 gene was introduced into porcine embryonic germ (EG) cells. Treatment of human serum did not affect the survival of hCD46-transgenic EG cells, whereas the treatment significantly reduced the survival of non-transgenic EG cells (p < 0.01). The transgenic EG cells presumably capable of alleviating HAR were transferred into enucleated oocytes. Among 235 reconstituted oocytes, 35 (14.9%) developed to the blastocyst stage. Analysis of individual embryos indicated that 80.0% (28/35) of embryos contained the transgene hCD46. The result of the present study demonstrates resistance of hCD46-transgenic EG cells against HAR, and the usefulness of the transgenic approach may be predicted by this cytolytic assessment prior to actual production of transgenic pigs. Subsequently performed EG cell nuclear transfer gave rise to hCD46-transgenic embryos. Further study on the transfer of these embryos to recipients may produce hCD46-transgenic pigs.
Authors:
Ji Young Won; Kwang Sung Ahn; Alice M Sorrell; Susa Shin; Soon Young Heo; Jee Hyun Kang; Jin-Ki Park; Won-Kyong Chang; Hosup Shim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-09
Journal Detail:
Title:  Zygote (Cambridge, England)     Volume:  17     ISSN:  0967-1994     ISO Abbreviation:  Zygote     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-01     Completed Date:  2009-06-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9309124     Medline TA:  Zygote     Country:  England    
Other Details:
Languages:  eng     Pagination:  101-8     Citation Subset:  IM    
Affiliation:
Department of Physiology, Dankook University School of Medicine, Cheonan, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified*
Antigens, CD46 / genetics*,  metabolism
Blastocyst / physiology*
Embryonic Development
Gene Transfer Techniques
Humans
Nuclear Transfer Techniques
Oocytes / physiology*
Swine / genetics*
Transgenes
Chemical
Reg. No./Substance:
0/Antigens, CD46; 0/CD46 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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