Document Detail

Cytological and ultrastructural investigation on osteoblastic and preosteoclastic cells grown in vitro in the presence of ipriflavone: preliminary results.
MedLine Citation:
PMID:  1422316     Owner:  NLM     Status:  MEDLINE    
The effects of ipriflavone on bone cells were studied in vitro on pre-osteoclastic (FLG 29.1) and osteoblast-like (Saos-2) cells grown for 48 h either separately or in co-cultures, with or without the addition of PTH. Histological, ultrastructural and histochemical (TRAP-activity demonstration) methods were used. The main results show that ipriflavone reduces replication of FLG 29.1 cells and inhibits TRAP production by these cells both in controls and in co-cultures treated with PTH. Moreover, it has a moderate stimulatory effect on proliferation of osteoblast-like cells and reduces the PTH-induced degenerative changes of Saos-2 cells. These results suggest that the inhibitory effect of ipriflavone on FLG 29.1 cells might be indirect and might be mediated by the osteoblast-like cells.
E Bonucci; G Silvestrini; P Ballanti; L Masi; A Franchi; L Bufalino; M L Brandi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bone and mineral     Volume:  19 Suppl 1     ISSN:  0169-6009     ISO Abbreviation:  Bone Miner     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-12-23     Completed Date:  1992-12-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8610542     Medline TA:  Bone Miner     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  S15-25     Citation Subset:  IM    
Department of Human Biopathology, University La Sapienza, Rome, Italy.
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MeSH Terms
Acid Phosphatase / metabolism
Cell Adhesion / drug effects
Cell Division / drug effects
Isoflavones / pharmacology*
Microscopy, Electron
Osteoblasts / drug effects*,  ultrastructure
Osteoclasts / drug effects*,  ultrastructure
Parathyroid Hormone / pharmacology
Tumor Cells, Cultured
Reg. No./Substance:
0/Isoflavones; 0/Parathyroid Hormone; 35212-22-7/ipriflavone; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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