Document Detail

Cytological evaluation of capsular tissue surrounding TCPL implant in adult rats.
MedLine Citation:
PMID:  8672694     Owner:  NLM     Status:  MEDLINE    
Inflammatory response and the subsequent fibrous capsule formation are often used as a screening method to determine biocompatibility of an implanted material. In this study, porous implants of tricalcium phosphate-lysine (TCPL) delivery devices were implanted intraperitoneally (i.p.) and subcutaneously (s.c.) using adult male rats as a model. The fibrous capsule surrounding the implant was studied histochemically to determine the resorbability rate of the devices. Fibrous capsular tissues were carefully dissected away from the capsule noting the tissue closest to the implanted material. Evaluation of the sections (5um, H&E) collected from various areas of the implants (n = 15 per group) revealed that: (1) regardless of the site of surgical implantation, all devices were encapsulated with fibrous tissue within three days post implantation and the thickness of the capsular tissue was found to be directly proportional to duration of implantation, (2) the thickness of fibrous tissues collected from i.p. site were significantly higher than those collected from s.c. site of implantation, (3) vascularity, macrophages, multinucleated giant cells and plasma cells at the TCPL implant-interface were evident after the first week, and the persistence of these cell types and progressive angiogenesis in subsequent weeks was apparent. The formation of distinct cell layers was remarkable in the fifth week phase, (4) SEM and radiographic analysis of retrieved devices showed a significant (P < 0.05) increase in the number of macropores (12 to 36 um2) in TCPL devices implanted s.c. than the number of macropores in TCPL devices implanted i.p. The mechanism of biodegradation process in an in vivo environment is not fully elucidated. This observation and previous observations led us to suggest that the site of surgical implantation is an instrumental key in designing TCPL delivery system.
H Benghuzzi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedical sciences instrumentation     Volume:  32     ISSN:  0067-8856     ISO Abbreviation:  Biomed Sci Instrum     Publication Date:  1996  
Date Detail:
Created Date:  1996-08-12     Completed Date:  1996-08-12     Revised Date:  2013-04-05    
Medline Journal Info:
Nlm Unique ID:  0140524     Medline TA:  Biomed Sci Instrum     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  81-6     Citation Subset:  IM    
Department of Health Sciences, School of Health Related Professions, University of Mississippi Medical Center, Jackson 39216, USA.
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MeSH Terms
Biocompatible Materials / toxicity
Calcium Phosphates / toxicity*
Ceramics / toxicity*
Dermatologic Surgical Procedures
Foreign-Body Reaction / pathology*
Lysine / toxicity*
Peritoneum / pathology,  surgery
Prostheses and Implants*
Rats, Sprague-Dawley
Skin / pathology
Reg. No./Substance:
0/Biocompatible Materials; 0/Calcium Phosphates; 0/Ceramics; 0/alpha-tricalcium phosphate; 0/monocalcium phosphate; 0/tetracalcium phosphate; 56-87-1/Lysine; 97Z1WI3NDX/calcium phosphate; L11K75P92J/dicalcium phosphate anhydrous

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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