Document Detail


Cytological dissection of sex chromosome heterochromatin of Drosophila hydei.
MedLine Citation:
PMID:  6173175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prophase chromosomes of Drosophila hydei were stained with 0.5 microgram/ml Hoechst 33258 and examined under a fluorescence microscope. While autosomal and X chromosome heterochromatin are homogeneously fluorescent, the entirely heterochromatic Y chromosome exhibits an extremely fine longitudinal differentiation, being subdivided into 18 different regions defined by the degree of fluorescence and the presence of constrictions. Thus high resolution Hoechst banding of prophase chromosomes provides a tool comparable to polytene chromosomes for the cytogenetic analysis of the Y chromosome of D. hydei. - D. hydei heterochromatin was further characterized by Hoechst staining of chromosomes exposed to 5-bromodeoxyuridine for one round of DNA replication. After this treatment the pericentromeric autosomal heterochromatin, the X heterochromatin and the Y chromosome exhibit numerous regions of lateral asymmetry. Moreover, while the heterochromatic short arms of the major autosomes show simple lateral asymmetry, the X and the Y heterochromatin exhibit complex patterns of contralateral asymmetry. These observations, coupled with the data on the molecular content of D. hydei heterochromatin, give some insight into the chromosomal organization of highly and moderately repetitive heterochromatic DNA.
Authors:
S Bonaccorsi; S Pimpinelli; M Gatti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chromosoma     Volume:  84     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1981  
Date Detail:
Created Date:  1982-04-12     Completed Date:  1982-04-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  391-403     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bisbenzimidazole
Chromosome Banding
Drosophila / genetics*
Female
Heterochromatin / ultrastructure*
Male
Microscopy, Fluorescence
Repetitive Sequences, Nucleic Acid
Sex Chromosomes / ultrastructure*
Chemical
Reg. No./Substance:
0/Heterochromatin; 23491-44-3/Bisbenzimidazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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