| Cytokinins in tRNA Obtained from Spinacia oleracea L. Leaves and Isolated Chloroplasts. | |
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MedLine Citation:
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PMID: 16660279 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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Cytokinin-active ribonucleosides have been isolated from tRNA of whole spinach (Spinacia oleracea L.) leaves and isolated spinach chloroplasts. The tRNA from spinach leaf blades contained: 6-(4-hydroxy-3-methyl-2-butenylamino)-9-beta-d-ribofuranosylpurine (cis and trans isomers), 6-(3-methyl-2-butenylamino)-9-beta-d-ribofuranosylpurine, and 6-(4-hydroxy-3-methyl-2-butenylamino)-2-methylthio-9-beta-d -ribofuranosylpurine (cis and trans isomers). A method for isolation of large amounts of intact chloroplasts was developed and subsequently used for the isolation of chloroplast tRNA. The chloroplast tRNA contained 6-(3-methyl-2-butenylamino)-9-beta-d-ribofuranosylpurine and 6-(4-hydroxy-3-methyl-2-butenylamino)-2-methylthio-9-beta-d -ribofuranosylpurine (the cis isomer only). The structures of these compounds were assigned on the basis of their chromatographic properties and mass spectra of trimethylsilyl derivatives which were identical with those of the corresponding synthetic compounds. The results of this study indicate that ribosylzeatin was present in spinach leaf tRNA, but absent from the purified chloroplast tRNA preparation. |
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Authors:
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H J Vreman; R Thomas; J Corse |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Plant physiology Volume: 61 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 1978 Feb |
Date Detail:
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Created Date: 2010-06-29 Completed Date: 2010-06-29 Revised Date: 2010-09-15 |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 296-306 Citation Subset: - |
Affiliation:
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Western Regional Research Center, United States Department of Agriculture, Agricultural Research Service, Berkeley, California 94710. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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