Document Detail

Cytokinetic studies of oral cancer cells using bromodeoxyuridine labelling in relation to factors influencing prognosis.
MedLine Citation:
PMID:  7627085     Owner:  NLM     Status:  MEDLINE    
Cytokinetics of 27 untreated oral squamous cell carcinomas were evaluated by in vitro bromodeoxyuridine (BrdU) labelling. A mononuclear cell suspension was prepared for the labelling using collagenase and DNase treatment. The labelled cells were visualised by immunofluorescence staining with an anti-BrdU monoclonal antibody. The labelling index (LI%) was calculated by determining the percentage of BrdU labelled cells. The LIs of the carcinomas ranged from 3.8 to 19.2%. The LIs of poorly-differentiated (HG3) tumours were 2-3-fold higher compared with those of well-differentiated (HG1) and moderately-differentiated (HG2) carcinomas. Results also showed statistically significant (P < 0.0005) increases in LIs from nuclear grade 1 (NG1) to nuclear grade 3 (NG3) carcinomas. Higher LIs were observed in stage III/IV (10.82 +/- 4.62; 10.36 +/- 4.90) than those in stage I (6.87 +/- 2.09) and II (7.14 +/- 1.87) carcinomas. A significant (P < 0.0005) difference in LI values was found between the patients with positive and negative lymph nodes. Good correlation (r = 0.77) was exhibited between the LI values and mitotic counts (MC) of the specimens. These results on oral cancer cell proliferation seem to have prognostic implications.
D Mukhopadhyay; R Chatterjee; R N Chakraborty
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of cancer. Part B, Oral oncology     Volume:  31B     ISSN:  0964-1955     ISO Abbreviation:  Eur. J. Cancer, B, Oral Oncol.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-09-06     Completed Date:  1995-09-06     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9214373     Medline TA:  Eur J Cancer B Oral Oncol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  32-6     Citation Subset:  D; IM    
Department of Tumour Virology, Chittaranjan National Cancer Institute, Calcutta, India.
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MeSH Terms
Carcinoma, Squamous Cell / pathology*
Cell Cycle
Microscopy, Fluorescence
Middle Aged
Mitotic Index
Mouth Neoplasms / pathology*
Reg. No./Substance:

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