| Cytokinetic studies of oral cancer cells using bromodeoxyuridine labelling in relation to factors influencing prognosis. | |
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MedLine Citation:
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PMID: 7627085 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cytokinetics of 27 untreated oral squamous cell carcinomas were evaluated by in vitro bromodeoxyuridine (BrdU) labelling. A mononuclear cell suspension was prepared for the labelling using collagenase and DNase treatment. The labelled cells were visualised by immunofluorescence staining with an anti-BrdU monoclonal antibody. The labelling index (LI%) was calculated by determining the percentage of BrdU labelled cells. The LIs of the carcinomas ranged from 3.8 to 19.2%. The LIs of poorly-differentiated (HG3) tumours were 2-3-fold higher compared with those of well-differentiated (HG1) and moderately-differentiated (HG2) carcinomas. Results also showed statistically significant (P < 0.0005) increases in LIs from nuclear grade 1 (NG1) to nuclear grade 3 (NG3) carcinomas. Higher LIs were observed in stage III/IV (10.82 +/- 4.62; 10.36 +/- 4.90) than those in stage I (6.87 +/- 2.09) and II (7.14 +/- 1.87) carcinomas. A significant (P < 0.0005) difference in LI values was found between the patients with positive and negative lymph nodes. Good correlation (r = 0.77) was exhibited between the LI values and mitotic counts (MC) of the specimens. These results on oral cancer cell proliferation seem to have prognostic implications. |
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Authors:
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D Mukhopadhyay; R Chatterjee; R N Chakraborty |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European journal of cancer. Part B, Oral oncology Volume: 31B ISSN: 0964-1955 ISO Abbreviation: Eur. J. Cancer, B, Oral Oncol. Publication Date: 1995 Jan |
Date Detail:
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Created Date: 1995-09-06 Completed Date: 1995-09-06 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9214373 Medline TA: Eur J Cancer B Oral Oncol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 32-6 Citation Subset: D; IM |
Affiliation:
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Department of Tumour Virology, Chittaranjan National Cancer Institute, Calcutta, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Bromodeoxyuridine Carcinoma, Squamous Cell / pathology* Cell Cycle Female Humans Immunohistochemistry Male Microscopy, Fluorescence Middle Aged Mitotic Index Mouth Neoplasms / pathology* Prognosis |
| Chemical | |
Reg. No./Substance:
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59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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