Document Detail


Cytokines profile in cystic fibrosis patients with nasal polyps.
MedLine Citation:
PMID:  20345072     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nasal polyposis often complicates the progress of patients with cystic fibrosis and there has been little study about the importance of cytokines in the polyps of such individuals. OBJECTIVE: To assess RNAm expression for interleukins 4, 5, 6, 8, GM-CSF and IFN-gamma by RT-PCR in eosinophilic and non-eosinophilic polyps of patients with cystic fibrosis. MATERIAL AND METHOD: A total of 124 persons were evaluated, of which thirteen patients with cystic fibrosis and nasosinusal polyps were selected--three were eosinophilic and ten were non-eosinophilic. The control group was composed of eleven individuals with normal otorhinolaryngological exam and the mean age was 18 years (3-57). The middle turbinate mucosa and nasal polyps were biopsied from the control group and the cystic fibrosis group respectively, and these were analyzed with RT-PCR. The middle turbinate mucosa was biopsied in the control group and in the cystic fibrosis group polyps that was analyzed to RT-PCR. The polyps of cystic fibrosis patients were also further anaylsed for subjected to a second biopsy in order to determine the percentage of eosinophils. IL-4, IL-5, IL-6, IL-8, IFN-gamma and GM-CSF transcriptions were analyzed. RESULTS: There was no difference in IL-5, IL-8 and GM-CSF when compared to the eosinophilic, non-eosinophilic and control groups (p>0.05). When compared to the eosinophilic and non-eosinophilic groups, higher IL-4 and IL-6 values (p=0.01 and p=0.01 respectively) were observed. When analyzed separately with the control group, IL-4 (p=0.01) expression was higher in the eosinophilic group, while IFN-gamma (p=0.03) was lower in the non-eosinophilic group. IL-5, IL-8, GM-CSF are non-specific cytokines present in the nasosinusal sinonasal polyps of cystic fibrosis patients. IL-4 and IL-6 are important mediators in the eosinophilic sinonasal polyps, while low IFN-gamma may be related to lower eosinophils in non-eosinophilic polyps.
Authors:
F B Nunes; C G Becker; H M G Becker; R N Araujo; R E S Guimar?es
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Revue de laryngologie - otologie - rhinologie     Volume:  130     ISSN:  0035-1334     ISO Abbreviation:  Rev Laryngol Otol Rhinol (Bord)     Publication Date:  2009  
Date Detail:
Created Date:  2010-03-29     Completed Date:  2010-06-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0414144     Medline TA:  Rev Laryngol Otol Rhinol (Bord)     Country:  France    
Other Details:
Languages:  eng     Pagination:  163-7     Citation Subset:  IM    
Affiliation:
Federal University of Minas Gerais Medical School, Department of Ophthalmology and Otorhinolaryngology, Minas Gerais, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Child
Child, Preschool
Cystic Fibrosis / complications,  immunology*
Cytokines / analysis*
Humans
Middle Aged
Nasal Polyps / etiology,  immunology*
Prospective Studies
Young Adult
Chemical
Reg. No./Substance:
0/Cytokines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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