| Cytokines profile in cystic fibrosis patients with nasal polyps. | |
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MedLine Citation:
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PMID: 20345072 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nasal polyposis often complicates the progress of patients with cystic fibrosis and there has been little study about the importance of cytokines in the polyps of such individuals. OBJECTIVE: To assess RNAm expression for interleukins 4, 5, 6, 8, GM-CSF and IFN-gamma by RT-PCR in eosinophilic and non-eosinophilic polyps of patients with cystic fibrosis. MATERIAL AND METHOD: A total of 124 persons were evaluated, of which thirteen patients with cystic fibrosis and nasosinusal polyps were selected--three were eosinophilic and ten were non-eosinophilic. The control group was composed of eleven individuals with normal otorhinolaryngological exam and the mean age was 18 years (3-57). The middle turbinate mucosa and nasal polyps were biopsied from the control group and the cystic fibrosis group respectively, and these were analyzed with RT-PCR. The middle turbinate mucosa was biopsied in the control group and in the cystic fibrosis group polyps that was analyzed to RT-PCR. The polyps of cystic fibrosis patients were also further anaylsed for subjected to a second biopsy in order to determine the percentage of eosinophils. IL-4, IL-5, IL-6, IL-8, IFN-gamma and GM-CSF transcriptions were analyzed. RESULTS: There was no difference in IL-5, IL-8 and GM-CSF when compared to the eosinophilic, non-eosinophilic and control groups (p>0.05). When compared to the eosinophilic and non-eosinophilic groups, higher IL-4 and IL-6 values (p=0.01 and p=0.01 respectively) were observed. When analyzed separately with the control group, IL-4 (p=0.01) expression was higher in the eosinophilic group, while IFN-gamma (p=0.03) was lower in the non-eosinophilic group. IL-5, IL-8, GM-CSF are non-specific cytokines present in the nasosinusal sinonasal polyps of cystic fibrosis patients. IL-4 and IL-6 are important mediators in the eosinophilic sinonasal polyps, while low IFN-gamma may be related to lower eosinophils in non-eosinophilic polyps. |
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Authors:
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F B Nunes; C G Becker; H M G Becker; R N Araujo; R E S Guimar?es |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Revue de laryngologie - otologie - rhinologie Volume: 130 ISSN: 0035-1334 ISO Abbreviation: Rev Laryngol Otol Rhinol (Bord) Publication Date: 2009 |
Date Detail:
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Created Date: 2010-03-29 Completed Date: 2010-06-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0414144 Medline TA: Rev Laryngol Otol Rhinol (Bord) Country: France |
Other Details:
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Languages: eng Pagination: 163-7 Citation Subset: IM |
Affiliation:
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Federal University of Minas Gerais Medical School, Department of Ophthalmology and Otorhinolaryngology, Minas Gerais, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Child Child, Preschool Cystic Fibrosis / complications, immunology* Cytokines / analysis* Humans Middle Aged Nasal Polyps / etiology, immunology* Prospective Studies Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Cytokines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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