Document Detail


Cytokine signalling in rat pulp interstitial fluid and transcapillary fluid exchange during lipopolysaccharide-induced acute inflammation.
MedLine Citation:
PMID:  16527857     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The dental pulp consists of loose connective tissue encased in rigid dentinal walls. Because of its topography the tissue has low interstitial compliance and limited capacity to expand during fluid volume changes. Due to limitations regarding access to interstitial fluid, basic knowledge on transcapillary fluid transport parameters is lacking for this organ. The scope of this project was dual: first we aimed at establishing a method for isolation of pulp interstitial fluid (IF), and second we applied the method in rats subjected to lipopolysaccharide (LPS)-induced endotoxaemia. The aim was to measure colloid osmotic pressure (COP) and pro-inflammatory cytokines in the pulp IF during acute inflammation. Fluid volumes and pulpal blood flow (PBF) were measured to obtain more information about microcirculatory changes that take place in this pulpitis model. By centrifugation of incisor pulp at 239 g we were able to extract fluid representative for IF. Pulp IF had a relative high control COP (approximately 83% of plasma COP) and was similar to plasma COP 3 h after LPS challenge. The pulp exhibited a high content of IF (0.60 +/- 0.03 ml (g wet weight)(-1)) and a vascular volume of 0.03 +/- 0.01 ml (g w.w.)(-1) No differences were observed in the distribution of fluid volumes after 1.5 and 3 h LPS exposure. PBF and systemic blood pressure dropped significantly after LPS administration. PBF remained low whereas systemic blood pressure was re-established during the 3-h period, implying organ dysfunction. There was a differential pattern of cytokine expression in pulp IF and serum with cytokines such as IL-1alpha, IL-1beta and TNF-alpha locally produced, whereas others such as IFN-gamma and IL-6 were produced systemically and probably spilled over to the pulp IF after LPS exposure. Our findings show that pulp IF can be isolated by centrifugation and that this method is useful when studying fluid balance and extracellular signalling mechanisms in the dental pulp in normal and pathological conditions.
Authors:
Athanasia Bletsa; Ellen Berggreen; Inge Fristad; Olav Tenstad; Helge Wiig
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-03-09
Journal Detail:
Title:  The Journal of physiology     Volume:  573     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-16     Completed Date:  2006-07-12     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  225-36     Citation Subset:  IM    
Affiliation:
Department of Biomedicine, Section for Physiology, Jonas Lies vei 91, N-5009 Bergen, Norway.
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Animals
Blood Pressure
Body Fluids / immunology,  metabolism
Capillary Permeability / immunology
Cytokines / blood,  metabolism*
Dental Pulp / blood supply,  immunology*,  metabolism
Extracellular Fluid / immunology,  metabolism
Female
Interferon-gamma / blood,  metabolism
Interleukin-1 / blood,  metabolism
Interleukin-2 / blood,  metabolism
Interleukin-6 / blood,  metabolism
Lipopolysaccharides / pharmacology
Osmotic Pressure
Pulpitis / immunology*,  metabolism
Rats
Rats, Wistar
Reproducibility of Results
Signal Transduction / immunology*
Tumor Necrosis Factor-alpha / metabolism
Chemical
Reg. No./Substance:
0/Cytokines; 0/Interleukin-1; 0/Interleukin-2; 0/Interleukin-6; 0/Lipopolysaccharides; 0/Tumor Necrosis Factor-alpha; 82115-62-6/Interferon-gamma
Comments/Corrections
Comment In:
J Physiol. 2006 May 15;573(Pt 1):2-3   [PMID:  16581853 ]

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