Document Detail

Cytokine secretion and latent herpes virus reactivation with 28 days of horizontal hypokinesia.
MedLine Citation:
PMID:  17571663     Owner:  NLM     Status:  MEDLINE    
INTRODUCTION: Hypokinesia is associated with spaceflight and prolonged illnesses and may lead to secondary immune deficiency.
METHODS: The distribution of immunocytes in whole blood, mitogen-induced cytokine secretion in vitro, Epstein-Barr virus (EBV) reactivation, and plasma cortisol levels were studied in 13 healthy volunteers subjected to a horizontal bed rest (BR) regime for 28 d. Samples were collected before the study, weekly during BR, and then 3-5 d after the regime ended. Additionally, subjects were treated with hydrocortisone on the 1st and 27th d of BR to simulate the hypercortisolemia that occurs during stress.
RESULTS: The factors of 28-d BR regime accompanied by acute hypercortisolemia significantly decreased the relative and absolute number of total lymphocytes, CD3+ T-cells, T-helper subset, and monocytes, but increased the percentage of the CD8+ T-cells, and NK cells at the 4th wk compared with the baseline. A significant decrease in mitogen-activated secretion of IL-2, IFN-gamma, TNF-beta, IL-6, and IL-10 was registered at the same interval. Also, secretion of IL-2 and IFN-gamma declined at the 2nd week of the BR regime. Secretion of IL-4 was significantly higher at the 2nd and 3rd weeks compared with the baseline. A significant increase in the shedding of EBV DNA in saliva was observed as early as the 3rd wk of BR.
CONCLUSIONS: Stress factors associated with BR significantly alter immune responsiveness in vitro and in vivo. Changes in the cytokine secretion and cytokine imbalance precede latent EBV reactivation. PHA/LPS-activated cytokine secretion in whole blood can be used as a test system for predicting latent virus activation.
Peter N Uchakin; Raymond P Stowe; Douglas Paddon-Jones; Brian W Tobin; Arny A Ferrando; Robert R Wolfe
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Aviation, space, and environmental medicine     Volume:  78     ISSN:  0095-6562     ISO Abbreviation:  Aviat Space Environ Med     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-18     Completed Date:  2007-07-24     Revised Date:  2014-07-17    
Medline Journal Info:
Nlm Unique ID:  7501714     Medline TA:  Aviat Space Environ Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  608-12     Citation Subset:  IM; S    
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MeSH Terms
Amino Acids, Essential / immunology,  metabolism
Anti-Inflammatory Agents / immunology,  pharmacology
Cytokines / drug effects,  secretion*
Dietary Supplements
Herpesvirus 4, Human / immunology*
Hydrocortisone / blood,  immunology
Immobilization / adverse effects*,  physiology*
Middle Aged
Saliva / virology
Space Simulation / adverse effects
Stress, Psychological / immunology,  virology
Virus Activation / immunology*
Virus Latency / immunology,  physiology
Grant Support
5 R01 GM 57295/GM/NIGMS NIH HHS; P30 AG024832/AG/NIA NIH HHS
Reg. No./Substance:
0/Amino Acids, Essential; 0/Anti-Inflammatory Agents; 0/Cytokines; WI4X0X7BPJ/Hydrocortisone

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