Document Detail

Cytokine reporter mice in immunological research: perspectives and lessons learned.
MedLine Citation:
PMID:  21070235     Owner:  NLM     Status:  MEDLINE    
Cytokines are soluble messenger molecules with important regulatory functions throughout the immune system. 'Cytokine reporter' strains express marker molecules under control of elements from cytokine genes allowing for easy identification of their cellular sources. Such systems are well-accepted tools for research of cytokine function. The value of these strains lies in the ability to perform experiments relying on identification and isolation of live cytokine-expressing cells, provided that the reporter faithfully reflects the proper cytokine mRNA and protein production. As more diverse cell subsets are defined by their cytokine expression, the field has adapted with the generation of more sophisticated strains. In this review we summarize the evolution of cytokine detection methods and give examples of knowledge gained using cytokine reporter mice for cell types expressing interferon-γ and interleukin-4, -10 and -17. We also discuss current options for generating such reporter strains and their potential pitfalls.
Andrew L Croxford; Thorsten Buch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2010-11-11
Journal Detail:
Title:  Immunology     Volume:  132     ISSN:  1365-2567     ISO Abbreviation:  Immunology     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-08     Completed Date:  2011-01-31     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
Institute of Experimental Immunology, Department of Pathology, University of Zurich, Zurich, Switzerland.
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MeSH Terms
Allergy and Immunology*
Cytokines / analysis,  biosynthesis,  genetics*,  immunology*
Genes, Reporter / genetics*
Mice, Transgenic
Promoter Regions, Genetic / genetics
T-Lymphocytes, Helper-Inducer / immunology
Reg. No./Substance:

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