Document Detail


Cytokine release in tissue-engineered epidermal equivalents after prolonged mechanical loading.
MedLine Citation:
PMID:  19908014     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prolonged mechanical loading of soft tissues may result in degeneration of these tissues, resulting in formation of pressure ulcers. The risk assessment of individuals might be improved by including measurements of the tissue response to mechanical loading. Cytokines, which are released by the top layer of the skin upon chemical irritation, might be used to determine the epidermal response to mechanical loading. This chapter describes methods to measure the release of cytokines IL-1 alpha, IL-1RA, and IL-8 from tissue-engineered epidermal equivalents in response to sustained mechanical loading. A custom-built loading device was used to apply load to the epidermal equivalents and the cytokines were measured using an enzyme-linked immunosorbent assay.
Authors:
Lisette H Cornelissen; Debbie Bronneberg; Susan Gibbs; Carlijn V C Bouten; Cees W J Oomens
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  585     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2009-11-12     Completed Date:  2010-01-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  335-44     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Cytokines / metabolism*
Enzyme-Linked Immunosorbent Assay
Epidermis / metabolism*
Humans
Interleukin 1 Receptor Antagonist Protein / metabolism
Interleukin-1alpha / metabolism
Interleukin-8 / metabolism
Tissue Engineering / instrumentation,  methods*
Chemical
Reg. No./Substance:
0/Cytokines; 0/Interleukin 1 Receptor Antagonist Protein; 0/Interleukin-1alpha; 0/Interleukin-8

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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