| Cytokine profiling of pancreatic fluid using the ePFT collection method in tandem with a multiplexed microarray assay. | |
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MedLine Citation:
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PMID: 21569776 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cytokines are secreted immunomodulating proteins involved in pancreatic stellate cell activation and propagation of fibrosis in chronic pancreatitis. We aim to show that cytokines can be identified from pancreatic fluid by (1) collecting pancreatic fluid with the ePFT method, (2) processing the fluid for cytokine-targeted microarray analysis, and (3) comparing cytokine profiles in pancreatic fluid of chronic pancreatitis (CP) patients and of chronic abdominal pain (CAP) controls. We endoscopically collected pancreatic fluid from patients with CP and those with CAP using the ePFT method. This fluid was subjected directly to a multiplexed cytokine protein microarray assay. Six patients (3 CP, 3 CAP) underwent a secretin-stimulated ePFT. The mean peak bicarbonate concentrations [meq/L] of the CP and CAP patients were 43 and 97, respectively. Statistically significant decreases in the cytokine concentrations of EGF, IP-10, eotaxin, IL-3, MIP-1a, IL-15, PDGF-AB/BB, and IL-1a were observed in the CP specimens (p<0.05). We have successfully identified differences in the abundance of cytokines in ePFT-collected pancreatic fluid with a multiplexed microarray assay comparing CP and CAP controls. Further targeted investigation of cytokines in ePFT-collected fluid will broaden our knowledge of pancreatic immune response and pathogenesis in chronic pancreatitis. |
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Authors:
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Joao A Paulo; Linda S Lee; Bechien Wu; Peter A Banks; Hanno Steen; Darwin L Conwell |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-05-01 |
Journal Detail:
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Title: Journal of immunological methods Volume: 369 ISSN: 1872-7905 ISO Abbreviation: J. Immunol. Methods Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-06-15 Completed Date: 2011-08-15 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 98-107 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Pathology, Children's Hospital Boston, Boston, MA, United States. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Abdominal Pain
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physiopathology Adult Chronic Disease Cytokines / analysis* Endoscopy* Female Humans Male Middle Aged Pancreatic Function Tests / methods* Pancreatic Juice / chemistry* Pancreatitis, Chronic / physiopathology Protein Array Analysis / methods* |
| Grant Support | |
ID/Acronym/Agency:
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1 F32 DK085835-01A1/DK/NIDDK NIH HHS; 1 R21 DK081703-01A2/DK/NIDDK NIH HHS; 5 P30 DK034854-24/DK/NIDDK NIH HHS; R21 DK081703-01A2/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cytokines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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