| Cytokine production by cord blood mononuclear cells stimulated with cows milk proteins in vitro: interleukin-4 and transforming growth factor beta-secreting cells detected in the CD45RO T cell population in children of atopic mothers. | |
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MedLine Citation:
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PMID: 12752590 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Food antigens from the maternal circulation may sensitize fetal T cells in utero and be an important determinant in the development of food allergy. METHODS: Here we have examined the spontaneous and recall response to cow's milk proteins of cord blood mononuclear cells (CBMC) of newborn children, using single cell ELISPOT assays. RESULTS: In term newborns, confirming previous studies, the spontaneous cytokine response of CBMC is dominated by IL-4, IL-5, IL-10, and as shown here for the first time, TGF-beta. For TGF-beta only, the response of samples from infants of atopic mothers was significantly lower than samples from infants of non-atopic mothers. In vitro stimulation of CBMC with bovine serum albumin, casein and beta-lactoglobulin resulted in a significant increase of all cytokine-secreting cells, again dominated by T helper type 2 (Th2) cytokines. There was a clear tendency for samples from infants of atopic mothers to have lower Th2 responses than samples from infants of non-atopic mothers, which was particularly significant for both IL-4 and TGF-beta. Spontaneous cytokine secreting cells were virtually absent in cord blood from infants < 34 weeks gestation, as were cows milk protein-induced responses, although they were readily detectable in samples from infants aged > 34 weeks. To explore whether the cytokine secreting cells were in the naive CD4+ CD45RA population or memory CD4+ CD45RO T cells, these subsets were purified by positive and negative selection and tested for spontaneous and cows milk protein-induced cytokine responses. Strikingly, although the responses were small, the CD45RO+ cells from children of atopic mothers showed significant spontaneous and antigen-specific IL-4 and TGF-beta responses, whereas the same population from infants of non-atopic mothers showed virtually no response. In addition CD45RA+ cells from infants of mothers with maternal atopy showed decreased IL-4 and TGF-beta responses, especially the latter. CONCLUSIONS: The cows milk antigen-specific IL-4 and TGF-beta responses preferentially seen in the memory cell subset of infants with a maternal history of atopy strongly suggests Th2 skewing to dietary antigens in utero. |
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Authors:
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A C Hauer; M Riederer; A Griessl; H Rosegger; T T MacDonald |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology Volume: 33 ISSN: 0954-7894 ISO Abbreviation: Clin. Exp. Allergy Publication Date: 2003 May |
Date Detail:
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Created Date: 2003-05-19 Completed Date: 2003-09-04 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8906443 Medline TA: Clin Exp Allergy Country: England |
Other Details:
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Languages: eng Pagination: 615-23 Citation Subset: IM |
Affiliation:
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Universitätsklinik fur Kinder- und Jugendheilkunde, Karl-Franzens-Universität, Graz, Austria. almuthe.hauer@kfunigraz.ac.at |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD45 / analysis CD4-Positive T-Lymphocytes / immunology Cells, Cultured Cytokines / biosynthesis* Female Fetal Blood / immunology* Humans Hypersensitivity, Immediate / immunology* Infant, Newborn Infant, Premature / immunology Interleukin-4 / biosynthesis Male Maternal-Fetal Exchange / immunology Milk Proteins / immunology* Pregnancy Pregnancy Complications / immunology T-Lymphocyte Subsets / immunology* Transforming Growth Factor beta / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Milk Proteins; 0/Transforming Growth Factor beta; 207137-56-2/Interleukin-4; EC 3.1.3.48/Antigens, CD45 |
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