Document Detail

Cytokine-induced restoration of differentiation and cell cycle arrest in v-Myc transformed U-937 monoblasts correlates with reduced Myc activity.
MedLine Citation:
PMID:  11236937     Owner:  NLM     Status:  MEDLINE    
Deregulated expression of the myc-family of oncogenes in hematopoietic and other cell types plays an important role in tumorigenesis, and results in increased proliferative potential and block of cellular differentiation. We have previously shown that IFN-gamma restores phorbol ester-induced differentiation and cell cycle arrest in v-myc transformed human U-937 monoblasts. To investigate whether other cytokine signals could also abrogate such a block, IL-1, IL-3, IL-4, IL-6, IL-7, IL-10, IL-11, LIF, oncostatin M, M-CSF, G-CSF and GM-CSF, and TGFbeta1, TNF-alpha, IFN-alpha were examined. We show that GM-CSF and IL-6, in combination with the phorbol ester 12-O-tetradecanoyl-phorbol acetate (TPA), restored differentiation and cell cycle arrest. In contrast, treatment by TGFbeta1 +/- TPA resulted in an efficient G1/G0 arrest, but did not appear to induce terminal differentiation. Restoration of differentiation and cell cycle arrest was accomplished despite maintained expression of the v-Myc protein. Our results show that the cytokine-induced signals reduced Myc-dependent transcription of an artificial target promoter/reporter gene construct, correlating in most, but not all, cases with decreased association of v- and c-Myc with its essential partner, Max. Thus, cytokine-induced signals may counteract the activity of deregulated Myc, and contribute to the normalization of differentiation, arrest in the G1/G0 phase of the cell cycle, or both.
F Oberg; S Wu; F Bahram; K Nilsson; L G Larsson
Related Documents :
21554547 - Guanylate cyclase activator yc-1 enhances trail-induced apoptosis in human epithelial o...
21472277 - The functional role of dendritic cells in atherogenesis (review).
11733227 - Oncogenic co-operation in beta-cell tumorigenesis.
21530647 - Pro-oxidants ameliorate radiation-induced apoptosis through activation of the calcium-e...
20178457 - Effects of pharmacological inhibition of nadph oxidase or inos on pro-inflammatory cyto...
24455767 - Spontaneous activation of rna-sensing pathways in autoimmune disease.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Leukemia     Volume:  15     ISSN:  0887-6924     ISO Abbreviation:  Leukemia     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-03-09     Completed Date:  2001-03-29     Revised Date:  2013-03-04    
Medline Journal Info:
Nlm Unique ID:  8704895     Medline TA:  Leukemia     Country:  England    
Other Details:
Languages:  eng     Pagination:  217-27     Citation Subset:  IM    
Department of Genetics and Pathology, University of Uppsala, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Cycle / physiology*
Cell Differentiation / drug effects,  physiology*
Cytokines / physiology*
Genes, myc*
Tetradecanoylphorbol Acetate / pharmacology
U937 Cells
Reg. No./Substance:
0/Cytokines; 16561-29-8/Tetradecanoylphorbol Acetate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Comparison of the L10M consolidation regimen to an alternative regimen including escalating methotre...
Next Document:  FGF-2 abnormalities in B cell chronic lymphocytic and chronic myeloid leukemias.