Document Detail


Cytokine gene-modified tumor cells for prophylactic and therapeutic vaccination: IL-2, IFN-gamma, or combination IL-2 + IFN-gamma.
MedLine Citation:
PMID:  9681248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Murine melanoma cells were engineered to express interleukin-2 (IL-2), interferon-gamma (IFN-gamma) or both cytokines at various dose levels by means of the adenovirus-enhanced transferrinfection (AVET) method. The gene-modified cells were tested for their potency to induce an antitumor immune response in two experimental settings with different tumor load. In a prophylactic vaccination model, both IL-2 and IFN-gamma showed a dose-dependent protection against tumor cell challenge in two melanoma models. In the therapeutic vaccination model, where mice with measurable tumors were treated, immunization with IL-2 or IFN-gamma gene-modified cells led to complete tumor regression in 30% or 20% of the tumor-bearing animals respectively. The combination of IL-2 + IFN-gamma resulted in complete tumor regression in up to 50% of the tumor-bearing mice.
Authors:
R Kircheis; Z Küpcü; G Wallner; E Wagner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytokines, cellular & molecular therapy     Volume:  4     ISSN:  1368-4736     ISO Abbreviation:  Cytokines Cell. Mol. Ther.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-10-08     Completed Date:  1998-10-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9713367     Medline TA:  Cytokines Cell Mol Ther     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  95-103     Citation Subset:  IM    
Affiliation:
Bender & Co GmbH, Vienna, Austria. Kircheis@Bender.co.at
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MeSH Terms
Descriptor/Qualifier:
Animals
Cancer Vaccines / administration & dosage*,  genetics
Disease Models, Animal
Female
Gene Therapy / methods
Interferon-gamma / genetics*
Interleukin-2 / genetics*
Melanoma / immunology,  prevention & control*,  therapy*
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
T-Lymphocytes, Cytotoxic / immunology
Transfection
Tumor Cells, Cultured
Vaccination
Chemical
Reg. No./Substance:
0/Cancer Vaccines; 0/Interleukin-2; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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