| Cytokine gene expression in response to Haemonchus placei infections in Nelore cattle. | |
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MedLine Citation:
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PMID: 20409640 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study aimed to evaluate the expression of a subset of cytokine genes in response to Haemonchus placei infections in Nelore cattle presenting different degrees of resistance to natural infections. One hundred weaned bulls, initially 11-12 months old, were evaluated and kept on the same pasture. Faecal and blood samples were collected for parasitological and immunological assays. The seven most resistant and the eight most susceptible animals were selected based on nematode faecal egg counts (FEC) and worm burden. Serum was collected to measure antibody titres, and abomasum and abomasal lymph node tissue samples were collected to analyse the expression of a subset of cytokine genes (IL-2, IL-4, IL-8, IL-12p35, IL-13, TNF-alpha, IFN-gamma, MCP-1, MCP-2, MUC-1) using real-time RT-PCR. Mast cells, eosinophils and globule leukocytes in the abomasal mucosa were enumerated, and IgA levels in the mucus were assessed. Gene expression analysis in the abomasal tissue indicated that IL-4 and IL-13 (TH2 cytokines) were up-regulated in the resistant group, whereas TNF-alpha (TH1/TH2 cytokine) was up-regulated in the susceptible group. In abomasal lymph nodes, IL-4 and IFN-gamma were up-regulated in the resistant and susceptible groups, respectively. In the resistant group, serum IgG1 levels were higher against antigens of H. placei infective larvae on days 14, 42, 70 and 84 and against antigens of H. placei adults on day 84 (P<0.05). The resistant group had higher mast cell counts in the abomasal mucosa than the susceptible group (P<0.05). These results indicate a protective TH2-mediated immune response against H. placei in the resistant group and a less protective TH1 response in the susceptible group. |
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Authors:
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L G Zaros; P A Bricarello; A F T Amarante; R A Rocha; F N J Kooyman; E De Vries; L L Coutinho |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-19 |
Journal Detail:
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Title: Veterinary parasitology Volume: 171 ISSN: 1873-2550 ISO Abbreviation: Vet. Parasitol. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-23 Completed Date: 2010-09-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7602745 Medline TA: Vet Parasitol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 68-73 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Escola Superior de Agricultura Luiz de Queiroz-ESALQ/USP, Departamento de Zootecnia, Laboratório de Biotecnologia Animal, Piracicaba, SP, Brazil. lgzaros@gmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Cattle Diseases / immunology, parasitology* Cytokines / blood, genetics, immunology* Feces / parasitology Gastrointestinal Diseases / immunology, parasitology, veterinary* Gene Expression Profiling / veterinary Gene Expression Regulation / immunology* Haemonchiasis / immunology, parasitology, veterinary* Haemonchus / genetics, immunology* Histocytochemistry / veterinary Immunoglobulins / blood Lymph Nodes / immunology, parasitology Male Parasite Egg Count / veterinary RNA / chemistry, genetics Reverse Transcriptase Polymerase Chain Reaction / veterinary |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Immunoglobulins; 63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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