| Cytokine and chemokine responses in serum and brain after single and repeated injections of lipopolysaccharide: Multiplex quantification with path analysis. | |
| | |
MedLine Citation:
|
PMID: 21704698 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Administration of the proinflammatory molecule lipopolysaccharide (LPS) alters transport rates for many peptides across the blood-brain barrier (BBB). We and others have previously shown that effects of LPS on BBB transport are highly dependent on the injection paradigm used, and timing of the study. Cytokine expression in both brain and serum compartments influence the BBB response to an inflammatory stimulus, and mediate changes in BBB transport. Here, we used multianalyte technology to simultaneously determine the responses of 13 cytokines and chemokines (G-CSF, GM-CSF, IL-1α, IL-1β, IL-6, IL-10, IL-13, IP-10, KC, MCP-1, MIP-1α, RANTES, and TNF-α) in brain and blood to single and repeated injections of LPS and path analysis to determine the major relations among these analytes. Major findings are: (1) in comparison to measurements taken from a time course after a single injection of LPS, the three injection regimen of LPS produced significantly higher levels in brain for G-CSF, IL-1α, IL-6, MCP-1, MIP-1α, and TNF and in serum for G-CSF, IL-6, and GM-CSF and (2) path analysis distinguished direct from indirect correlations between analyte pairs, with MCP-1, IL-6, G-CSF, and KC mediating relations among these cytokines both within and between serum and brain compartments. These results suggest that potentiation of cytokine levels in brain and serum compartments could play important roles in the regulation of BBB transport, and that our novel application of an established statistical method can be used to assess direct correlations within multiplexed datasets. |
| | |
Authors:
|
Michelle A Erickson; William A Banks |
Related Documents
:
|
10506188 - Mitochondrial phospholipid hydroperoxide glutathione peroxidase suppresses apoptosis me... 15211078 - Mitochondria-targeting drug oligomycin blocked p-glycoprotein activity and triggered ap... 11889418 - The role of mitochondria in ischemia/reperfusion injury. 19479378 - Inside an enigma: do mitochondria contribute to cell death in drosophila? 12946828 - Interactions between the enteric nervous system and the immune system: role of neuropep... 9458368 - Alterations in differentiation and apoptosis induced by bufalin in cjun overexpressing ... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-6-17 |
Journal Detail:
|
Title: Brain, behavior, and immunity Volume: - ISSN: 1090-2139 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-6-27 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8800478 Medline TA: Brain Behav Immun Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
|
Geriatrics Research Education and Clinical Center, Veterans Affairs Puget Sound Health Care System, United States; Department of Pharmacological and Physiological Sciences, Saint Louis University School of Medicine, United States. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Deficiency of corticotropin-releasing hormone type-2 receptor alters sleep responses to bacterial li...
Next Document: Changed preference for sweet taste in adulthood induced by perinatal exposure to bisphenol A-A proba...