Document Detail


Cytokine secretion pattern in treatment of lymphocytes of multiple sclerosis patients with fumaric acid esters.
MedLine Citation:
PMID:  21510778     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study was performed to investigate the effects of dimethylfumarate (DMF) and methylhydrogen fumarate (MHF) on the cytokine pattern of peripheral blood mononuclear cells (PBMCs) of multiple sclerosis (MS) patients. The PBMCs from patients and healthy controls were stimulated with myelin basic protein (MBP) or phytohemagglutinin (PHA) and cultured in the presence of DMF and MHF. The percentage of CD4+IL-4+ and CD4+IFN-γ+ cells was determined by means of intracellular cytokine staining. CD4+IL-4+ cells were significantly increased in the presence of DMF and MHF when PBMCs were stimulated by MBP (P < 0.003). The same significant result was obtained by PHA stimulation (P < 0.049). In terms of CD4+IFN-γ+ cells, the percentage of cells did not significantly differ between the cultures stimulated with MBP or PHA in the presence and absence of the drugs. Results of MBP stimulation in control group also showed a significant increase in CD4+IL-4+ cells in the presence of DMF and MHF. In comparison between patient and control groups, no statistically significant changes were observed. In conclusion, both DMF and MHF effectively increased IL-4 production, whereas they did not significantly change IFN-γ level, indicating the role of these drugs in increasing the production of beneficial cytokines such as IL-4.
Authors:
Samaneh Zoghi; Zahra Amirghofran; Alireza Nikseresht; Nahid Ashjazadeh; Eskandar Kamali-Sarvestani; Nahid Rezaei
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-21
Journal Detail:
Title:  Immunological investigations     Volume:  40     ISSN:  1532-4311     ISO Abbreviation:  Immunol. Invest.     Publication Date:  2011  
Date Detail:
Created Date:  2011-06-23     Completed Date:  2012-04-09     Revised Date:  2013-04-01    
Medline Journal Info:
Nlm Unique ID:  8504629     Medline TA:  Immunol Invest     Country:  England    
Other Details:
Languages:  eng     Pagination:  581-96     Citation Subset:  IM    
Affiliation:
Immunology Department, Shiraz University of Medical Sciences, Shiraz, Iran.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
CD4-Positive T-Lymphocytes / cytology,  drug effects,  immunology,  metabolism
Cell Count
Cell Survival / drug effects
Cytokines / secretion*
Female
Fumarates / pharmacology*
Humans
Interferon-gamma / metabolism
Interleukin-4 / metabolism
Leukocytes, Mononuclear / cytology,  drug effects,  immunology,  metabolism
Lymphocyte Activation / drug effects,  immunology
Lymphocytes / cytology,  drug effects*,  immunology,  secretion*
Male
Maleates / pharmacology
Middle Aged
Multiple Sclerosis / immunology,  metabolism*
Myelin Basic Protein / immunology,  pharmacology
Phytohemagglutinins / pharmacology
Th1 Cells / cytology,  drug effects,  immunology,  metabolism
Th2 Cells / cytology,  drug effects,  immunology,  metabolism
Young Adult
Chemical
Reg. No./Substance:
0/Cytokines; 0/Fumarates; 0/IL4 protein, human; 0/MBP protein, human; 0/Maleates; 0/Myelin Basic Protein; 0/Phytohemagglutinins; 207137-56-2/Interleukin-4; 498-23-7/citraconic acid; 82115-62-6/Interferon-gamma; FO2303MNI2/dimethyl fumarate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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