| Cytokine Profiles in Peripheral Blood of Children and Adults with Crohn's Disease: A Pilot Study. | |
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MedLine Citation:
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PMID: 22167015 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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ABSTRACT: Increasing evidence suggeststhat cytokine dysregulation in T-helper 1 and T-helper 2 (Th1/Th2) subsets contributes to the pathogenesis of Crohn's disease (CD). This pilot studyexamines the hypothesis that cytokine profiles differ between pediatric and adult CD patients. Production of Th1 cytokines interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) and of Th2 cytokines interleukin-4 (IL-4) and interleukin-6 (IL-6) were analyzed in peripheral blood of patients with CD and healthy controls (n = 20) using flow cytometryafter in vitro stimulation. In both pediatric and adult subjects, frequencies of TNF-α CD4+ T cells were higher in CD patients than in controls (p = 0.009, p = 0.047, respectively). Percentagesof cells expressing IL-4 were slightly increased (p = 0.036) while those for IFN-γ were decreased (p = 0.009) in pediatric CD patients compared with controls. As expected, the overall production of Th1 cytokines was higher in adults compared with pediatric subjects. When memory CD4+CD45RO+ T cells were considered, lower IFN-γ expressionwasobservedin pediatric CD subjects compared with controls (p = 0.009), matching the trend seen in the general CD4+ T cell population. The percentage of CD4+CD45RO+ T cells were increased in adult CD patients compared with pediatric CD patients (p = 0.016). This study describes a peripheral blood Th1/Th2 cytokine imbalance in CD and suggests different immunological mechanisms between children and adults in disease pathogenesis. |
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Authors:
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Seung Pak; Nina Holland; Elizabeth A Garnett; Elizabeth Mileti; Uma Mahadevan; Rachel Beckert; Bittoo Kanwar; Melvin B Heyman |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-12 |
Journal Detail:
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Title: Journal of pediatric gastroenterology and nutrition Volume: - ISSN: 1536-4801 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8211545 Medline TA: J Pediatr Gastroenterol Nutr Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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*School of Public Health, University of California, Berkeley, CA †Departments of Pediatrics ‡Medicine, University of California, San Francisco, CA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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