Document Detail

Cytokeratin profile in exfoliative cytology of smokers.
MedLine Citation:
PMID:  22125842     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To compare the expression of cytokeratins (CKs) 6, 16, 19 and pan-cytokeratin (PAN) in oral mucosa cells between smokers and nonsmokers to determine the proliferative activity and expression indicative of a potential for malignant transformation.
STUDY DESIGN: Smears were obtained from the left lateral border of the tongue with a cytobrush from 25 smokers and 20 nonsmokers seen at the clinics of São José dos Campos Dental School, São Paulo State University, São José dos Campos, São Paulo, Brazil, and processed for immunohistochemistry. Conventional microscopy was used for qualitative analysis. Proportions were compared statistically by the z-test and Fisher's exact test.
RESULTS: The expression of CK6 (p = 0.002), CK16 (p = 0.003), CK19 (p = 0.0001) and PAN (p = 0.008) was higher in oral mucosa smears from smokers compared to nonsmokers.
CONCLUSION: The expression of CK6 and CK16 demonstrated increased epithelial proliferation in the oral mucosa of smokers, and expression of CK19 indicated alterations in epithelial maturation. The expression of PAN indicates the need for the investigation of other types of CK in further studies.
Celina Faig Lima; Sabrina Ferreira Pinotti Leite; Yasmin Rodarte Carvalho; Luiz Antonio Guimarães Cabral; Ivan Balducci; Janete Dias Almeida
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology     Volume:  33     ISSN:  0884-6812     ISO Abbreviation:  Anal. Quant. Cytol. Histol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-11-30     Completed Date:  2011-12-21     Revised Date:  2014-11-05    
Medline Journal Info:
Nlm Unique ID:  8506819     Medline TA:  Anal Quant Cytol Histol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  19-24     Citation Subset:  IM    
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MeSH Terms
Cell Transformation, Neoplastic / metabolism,  pathology
Keratin-16 / analysis,  metabolism*
Keratin-19 / analysis,  metabolism*
Keratin-6 / analysis,  metabolism*
Middle Aged
Mouth Mucosa / metabolism*,  pathology
Smoking / metabolism*,  pathology
Young Adult
Reg. No./Substance:
0/Keratin-16; 0/Keratin-19; 0/Keratin-6

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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