Document Detail

Cytokeratin 18 is a specific marker of bovine intestinal microfold (M) cell.
MedLine Citation:
PMID:  21193527     Owner:  NLM     Status:  Publisher    
Microfold (M) cells in the follicle associated epithelium (FAE) of Peyer's patches have an important role in mucosal immune responses. A primary difficulty for investigations of bovine M cells is the lack of a specific molecular marker. In order to identify such a marker we investigated the expression of several kinds of intermediate filament proteins using calf Peyer's patches. The expression patterns of cytokeratin (CK) 18 in jejunal and ileal FAE were very similar to the localization pattern of M cells recognized by scanning electron microscopy (SEM). Mirror sections revealed that jejunal CK18-positive cells had irregular and sparse microvilli, as well as pocket like structures containing lymphocytes, typical morphological characteristic of M cells. However, CK18-negative cells had regular and dense microvilli on their surface, typical of the morphology of enterocytes. In contrast, CK20 immunoreactivity was detected in almost all villous epithelial cells and CK18-negative cells in the FAE. CK18-positive proliferating transit-amplifying cells in the crypt exchanged CK18 for CK20 above the mouth of the crypt and after moving to the villi, however, CK18-positive M cells in the crypt continued their expression of CK18 during movement to the FAE region. TUNEL-positive apoptotic cells were specifically detected at the apical region of villi and FAE in the jejunum and ileum, and all were also stained for CK20. These data indicate that CK18 may be a molecular marker for bovine M cells in FAE, and that M cells may trans-differentiate to CK20-positive enterocytes and die by apoptosis in the apex of the FAE.
Tetsuya Hondo; Takashi Kanaya; Ikuro Takakura; Hitoshi Watanabe; Yu Takahashi; Yuya Nagasawa; Shunsuke Terada; Shyuichi Ohwada; Kouichi Watanabe; Haruki Kitazawa; Michael T Rose; Takahiro Yamaguchi; Hisashi Aso
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-30
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  -     ISSN:  1522-1547     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-1-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
1Graduate School of Agricultural Science, Tohoku University.
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