| Cytogenetics of multiple myeloma: interpretation of fluorescence in situ hybridization results. | |
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MedLine Citation:
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PMID: 12648063 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cytogenetic picture in multiple myeloma (MM) is highly complex, from which non-random numerical and structural chromosomal changes have been identified. Specifically, translocations involving the immunoglobulin heavy chain gene (IGH) at 14q32 and either monosomy or deletions of chromosome 13 have been reported in a significant number of patients from both cytogenetic and interphase fluorescence in situ hybridization (FISH) studies. Importantly, these abnormalities of chromosome 13 have recently been associated with a poor prognosis. In view of the highly complex nature of the karyotypes in MM patients, interphase FISH results may be difficult to interpret. In this study, cytogenetics and/or interphase FISH were carried out on bone marrow samples or purified plasma cells from 37 MM patients. Abnormal karyotypes, characterized by multiplex FISH (M-FISH) were found in 11 patients, all of which were highly complex. Interphase FISH revealed translocations involving the IGH locus in 16 (43%) patients. The IGH/cyclin D1 (CCND1) gene fusion characteristic of the translocation, t(11;14)(q13;q32), was seen in 12 (32%) of these patients and other rearrangements of IGH in four (11%) patients. Fourteen patients had additional copies of chromosome 11. Twenty patients (54%) had 13q14 deletions, 10 of whom also had t(11;14) or another IGH translocation. By comparing cytogenetic and FISH results, this study has revealed that significant chromosomal abnormalities might be hidden within highly complex karyotypes. Therefore, extreme caution is required in the interpretation of interphase FISH results in MM, particularly in relation to certain abnormalities, such as 13q14 deletions, which have an impact on prognosis. |
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Authors:
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Christine J Harrison; Helen Mazzullo; Kan L Cheung; Gareth Gerrard; G Reza Jalali; Atul Mehta; David G Osier; Kim H Orchard |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: British journal of haematology Volume: 120 ISSN: 0007-1048 ISO Abbreviation: Br. J. Haematol. Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-03-21 Completed Date: 2003-08-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372544 Medline TA: Br J Haematol Country: England |
Other Details:
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Languages: eng Pagination: 944-52 Citation Subset: IM |
Affiliation:
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Department of Haematology, Royal Free Medical School, London. harrison@soton.acuk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Case-Control Studies Chromosome Aberrations* Chromosomes, Human, Pair 11 Chromosomes, Human, Pair 13 Chromosomes, Human, Pair 14 Cytogenetic Analysis Female Gene Deletion Gene Rearrangement Humans Immunoglobulin Heavy Chains / genetics In Situ Hybridization, Fluorescence Interphase Karyotyping Male Metaphase Middle Aged Multiple Myeloma / genetics* Prognosis Translocation, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin Heavy Chains |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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