Document Detail


Cytogenetic studies in three Pimelodella meeki populations (Pisces, Pimelodidae) from Tibagi River basin (Brazil).
MedLine Citation:
PMID:  15475398     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We analyzed cytogenetically specimens of Pimelodella meeki from Tibagi River at Limoeiro (LM) and from two tributaries, Couro do Boi (CB) and Gabriel da Cunha (GC) Rivers. All specimens presented 2n=46 chromosomes, which were the karyotypes composed by 15 pairs metacentric (M) + 6 pairs submetacentric (SM) + 2 pairs subtelocentric (ST). In specimens of GC, CB, and LM, the results of analyses of the nucleolus organizer regions (NORs), done by means of AgNO3 and CMA3 staining, showed that they are identical, located in terminal position on the short arm of a SM chromosome pair, and they were observed to be a size heteromorphism in some metaphase plates. FISH with 18S rDNA probe yielded evidence for these regions but not for the size variation, indicating that they are not due to a greater number of NOR cistrons in one of the homologue chromosomes. An interesting characteristic of these regions is that they could appear divided in blocks, as evidenced by all the techniques. This work makes clear the necessity for more deeply systematic studies, because of the confused taxonomic situation of the genus Pimelodella.
Authors:
A P Vidotto; A C Swarça; A S Fenocchio; A L Dias
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of heredity     Volume:  95     ISSN:  0022-1503     ISO Abbreviation:  J. Hered.     Publication Date:    2004 Nov-Dec
Date Detail:
Created Date:  2004-10-11     Completed Date:  2004-12-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375373     Medline TA:  J Hered     Country:  United States    
Other Details:
Languages:  eng     Pagination:  517-20     Citation Subset:  IM    
Affiliation:
Universidade Estadual de Londrina, Depto. de Biologia Geral, Londrina, PR, Brazil, 86051-970, Caixa Postal 6001.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brazil
Chromomycin A3
Cytogenetic Analysis
DNA Probes
DNA, Ribosomal
Female
Fishes / classification,  genetics*
Karyotyping
Male
Nucleolus Organizer Region
Silver Staining
Chemical
Reg. No./Substance:
0/DNA Probes; 0/DNA, Ribosomal; 7059-24-7/Chromomycin A3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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